AI Article Synopsis
- The study aimed to investigate if levels of hypermethylated RASSF1A DNA in maternal plasma could indicate pre-eclampsia in pregnancies.
- Maternal plasma and placental tissues from both pre-eclamptic women and normotensive controls were analyzed using real-time PCR to measure RASSF1A levels, with actin as a control.
- Results showed that hypermethylated RASSF1A concentrations were significantly higher in the plasma of pre-eclamptic women, suggesting it could serve as a reliable marker for this condition.
Article Abstract
Objective: To study if quantitative aberrations in circulating placental-derived hypermethylated RASSF1A DNA in maternal plasma are associated with pre-eclamptic pregnancies.
Method: Maternal plasma and placental tissues from third-trimester pre-eclamptic women and gestational-age matched normotensive controls were studied. Real-time PCR was performed to quantify RASSF1A concentrations before and after methylation-sensitive restriction digestion in a duplex assay, where ss-actin concentrations were quantified as an internal control to confirm complete enzyme digestion.
Results: The median concentrations of hypermethylated RASSF1A were 4.3-fold higher in maternal plasma of pre-eclamptic subjects than in controls. There was no significant difference between the extent of RASSF1A hypermethylation in placental tissues obtained from pre-eclamptic and control pregnancies.
Conclusion: This study demonstrated the potential utility of hypermethylated RASSF1A sequences in maternal plasma as a gender- and polymorphism-independent marker for pre-eclampsia.
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| http://dx.doi.org/10.1002/pd.1897 | DOI Listing |
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