AI Article Synopsis
- Bone marrow-derived mesenchymal stem cells (MSCs) are versatile cells capable of differentiating into bone and fat cells, but their numbers and characteristics may vary with the age of the donor.
- The study aimed to analyze how aging affects the composition and differentiation abilities of MSCs using a rat model, employing methods like flow cytometry and microscopy for detailed examination.
- Findings revealed that while there were age-related changes in the quantity of MSCs and bone marrow composition, the actual phenotype and differentiation capabilities of the MSCs remained unchanged, highlighting the importance of considering donor age in MSC research.
Article Abstract
Bone marrow (BM) derived mesenchymal stem cells (MSC) are pluripotent cells which can differentiate into osteogenic, adipogenic and other lineages. In spite of the broad interest, the information about the changes in BM cell composition, in particularly about the variation of MSC number and their properties in relation to the age of the donor is still controversial. The aim of this study was to investigate the age associated changes in variations of BM cell composition, phenotype and differentiation capacities of MSC using a rat model. Cell populations were characterized by flow cytometry using light scattering parameters, DNA content and a set of monoclonal antibodies. Single cell analysis was performed by conventional fluorescent microscopy. In vitro culture of MSC was established and their phenotype and capability for in vitro differentiation into osteogenic and adipogenic cells was shown. Age related changes in tibiae and femurs, amount of BM tissue, BM cell composition, proportions of separated MSC and yield of MSC in 2 weeks of in vitro culture were found. At the same time, neither change in phenotype no in differentiation capacities of MSC was registered. Age-related changes of the number of MSC should be taken into account whenever MSC are intended to be used for investigations.
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