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Radioiodination of mouse anti-III beta-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies. | LitMetric
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Radioiodination of mouse anti-III beta-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies.

Helena Svecová Veronika Kleinová Daniel Seifert Hana Chaloupková Jörg Bäurle Karel Kranda Vlastimil Král Miroslav Fiser

Appl Radiat Isot

Nuclear Physics Institute of the ASCR, v.v.i., CZ-250 68 Rez near Prague, Czech Republic.

Published: March 2008

The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125 I in order to develop new imaging agents against the specific neuronal marker III beta-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.

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http://dx.doi.org/10.1016/j.apradiso.2007.09.005DOI Listing

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