AI Article Synopsis
- The study aimed to explore how cytomegalovirus (CMV) infection impacts the expression of matrix metalloproteinase-2 (MMP-2) in human endothelial cells.
- Human umbilical vein endothelial cells were infected with CMV and the levels of MMP-2 mRNA and activity were measured at various times post-infection.
- Results showed that MMP-2 expression and activity remained stable at 6 hours post-infection, but significantly increased at 12 and 24 hours, suggesting that CMV may play a role in atherosclerosis through this up-regulation of MMP-2.
Article Abstract
Objective: To investigate the effect of cytomegalovirus infection on expression of matrix metalloproteinase-2 in human endothelial cells.
Methods: Human umbilical vein endothelial cells were cultured in vitro. Cells between 3-6 passages were infected with cytomegalovirus for different time. Expression of matrix metalloproteinase-2 mRNA was analyzed by reverse transcription-polymerase chain reaction, matrix metalloproteinase-2 activity was detected by gel zymography.
Results: Expression of matrix metalloproteinase-2 mRNA and its activity 6 hours after infection was almost equal to control, and was greatly enhanced 12 and 24 hours after infection.
Conclusion: Cytomegalovirus infection up-regulates expression of matrix metalloproteinase-2 in human endothelial cells. It might be one of the mechanisms that cytomegalovirus is involved in atherosclerosis.
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