AI Article Synopsis
- The study investigated the effects of low-dose cisplatin on human squamous cell carcinoma cells, revealing its influence on various gene expressions related to cell processes.
- Out of 30,000 genes analyzed, 42 showed significant changes in expression levels, notably those linked to apoptosis, cell cycle regulation, and DNA repair.
- Inhibiting the top 5 altered genes reduced apoptosis without impacting the overall cytotoxic effect of cisplatin, suggesting potential indirect effects and implications for drug resistance.
Article Abstract
Background: Substantial evidence has disclosed that some cytotoxic agents have complex activities in influencing signal transduction pathways in cells.
Materials And Methods: cDNA microarray analysis was performed after exposing a human squamous cell carcinoma cell line, RERF-LC-AI, to low-dose cisplatin for 5 days. Up-regulated gene expressions were suppressed by small interfering RNA to investigate phenotypic alteration of the cells.
Results: Among 30,000 genes screened, 42 genes showed increases or decreases in expression of more than 2-fold with cisplatin treatment. They included genes with functions involved in apoptosis, cell cycle regulation and DNA metabolism/repair. Suppression of the 5 most significantly altered genes by small interfering RNA resulted in partly reduced apoptosis without altering cytotoxicity of cisplatin.
Conclusion: Besides direct cytotoxic effects on cells, cisplatin may have indirect effects involving drug resistance, and synergistic effects with other agents.
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