AI Article Synopsis
- The major capsid protein of the lymphocystis disease virus (LCDV-rc) from the fish Rachycentron canadum was amplified and analyzed to understand its genetic makeup.
- A 457bp DNA core fragment was successfully amplified using specially designed primers, and the complete sequence was reconstructed using inverse PCR.
- The study found that the gene has an open reading frame of 1380bp, encoding a protein of 459 amino acids, and phylogenetic analysis confirmed that LCDV-rc is closely related to other Lymphocystis viruses, classifying it as Lymphocystivirus.
Article Abstract
The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadum (LCDV-rc) was amplified and analysed. The 457bp DNA core fragment was amplified with the degenerate primers designed according to the conserved sequences of MCP gene of iridoviruses, then the flaking sequences adjacent to the core region were amplified by inverse PCR, and the complete sequence was obtained by combining all of them. The open reading frame of the gene is 1380bp in length, encoding a putative protein of 459 aa with molecular weight 51.12 kD and pI 6.87. Constructing the phylogenetic tree for comparing the MCP amino acid of iridoviruses, the results indicated that LCDV-rc is most homologous to the other Lymphocystis viruses and all of them constitute a branch. Accordingly LCDV-rc is identified as Lymphocystivirus.
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