Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE.

J Environ Sci (China)

Department of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Published: January 2008

AI Article Synopsis

  • * Researchers isolated two cadmium-resistant strains identified as Bacillus cereus and Enterobacter cloacae, assessing their characteristics through various methods.
  • * E. cloacae exhibited greater resistance to heavy metals and a broader range of antibiotic resistance compared to B. cereus, highlighting its potential for bioremediation.

Article Abstract

This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing. We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing, China, by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain, and found two dominant strains in the DGGE profile. Using special culture media, we isolated two strong cadmium-resistant bacterial strains. On the basis of morphological, physiological, and biochemical characteristics, BIOLOG, and 16S rDNA sequencing, the two strains were identified as Bacillus cereus and Enterobacter cloacae. Minimal inhibitory concentrations (MICs) of heavy metals for the bacteria were determined. E. cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B. cereus.

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http://dx.doi.org/10.1016/s1001-0742(07)60181-8DOI Listing

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