AI Article Synopsis
- The study investigated the effectiveness of intra-bone marrow (IBM) infusion of allogeneic hematopoietic stem cells in reducing graft-versus-host disease (GVHD) in mice.
- Female C57BL/6 mice were exposed to total body irradiation and then received stem cells from mobilized male BALB/c mice, with results showing that the IBM group experienced significantly lower GVHD incidence and severity compared to the tail vein injection group.
- The findings suggest that IBM infusion improves stem cell homing and reduces GVHD in allogeneic transplant scenarios.
Article Abstract
This study was aimed to explore whether the graft-versus-host disease (GVHD) could be alleviated by intra-bone marrow (IBM) infusion of allogeneic hematopoietic stem cells. Female C57BL/6 mice as recipients received total body irradiation (TBI) 4 Gy on day 0, followed by injection of peripheral hematopoietic stem cells (1 x 10(7)) from mobilized male BALB/c with granulocyte-colony stimulating factor (rhG-CSF), and cyclophosphamide (200 mg/kg) was injected intraperitoneally two days later. The results showed that the incidence and severity of GVHD were more low and alleviative in group IBM-PBSCT than that in group TV-PBSCT (p < 0.05). Y chromosome of donor mice could be detected in the bone marrow of recipient mice. It is concluded that the method of intra-bone marrow infusion is superior to injection via the tail vein in the engraftment of hematopoietic stem cells in terms of stem cell homing while the frequency and severity of GVHD in allogeneic mice decrease.
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