[The effects of nm23-H1 gene cell transfection on abilities of invasion, adhesion and chemotherapeutic sensitivity of bladder cancer T-24 cell line].

Objective: To investigate the transfection and expression of nm23-H1 gene in human bladder cancer cell line T-24, and to find out whether nm23-H1 could affect the metastases ability and the chemotherapeutic sensitivity of T-24 cell line.

Methods: By electroporation way, the pCMV-Bam-neo-nm23-H1 plasmid was transferred into bladder cancer line T-24, and the nm23-H1 vector-producer cell line T-24/nm23-H1 was isolated with G418 selection. The expression of nm23-H1 gene q,as detected by immunohistochemistry. The T-24 invasion, adherence and migration abilities between transfected and non-transfected cell lines were detected by transwell-room and wash techniques. The difference or change to the sensitivity of chemotherapeutic drug before and after cell transfected was evaluated by MTT.

Results: The stable expression of nm23-H1 was obtained by pCMV-Bam-Neo system with G418. The expression of nucleoside diphosphate kinase-A (NDPKA) was observed for significant difference between transfected and non-transfected T-24 cell lines; The invasive, adherence and migration abilities of transfected T-24 cells were decreased more than those of non-transfected T-24 (P < 0. 05); The chemo-sensitivity of transfected T-24 line cells to cis-diamminedichloroplatinum (CDDP) increased significantly.

Conclusion: nm23-H1 gene can inhibit the metastasis of T-24 cell line and increase the chemo-sensitivity of T-24 cell line to CDDP significantly.