AI Article Synopsis
- A calcium-activated chloride current (IClCa) is present in medium-sized sensory neurons of the dorsal root ganglion (DRG) and increases after sciatic nerve injury.
- Researchers used RT-PCR to study the gene expression of potential IClCa-inducing molecules in the DRG.
- Results indicate mBest1 and Tweety2 genes as strong candidates for contributing to the injury-induced IClCa in DRG neurons.
Article Abstract
Objective A calcium-activated chloride current (IClCa) has been observed in medium-sized sensory neurons of the dorsal root ganglion (DRG). Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule(s) underlying this current. Methods Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have IClCa current inducing properties. Results We showed the detection of transcripts representing several members of these families, i.e. chloride channel calcium-activated (CLCA), Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury. Conclusion Our analysis revealed that that mBest1 and Tweety2 appear as the best candidates to play a role in the injury-induced IClCa in DRG neurons.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5550578 | PMC |
| http://dx.doi.org/10.1007/s12264-007-0044-8 | DOI Listing |
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