AI Article Synopsis
- The authors introduce a straightforward image-based technique for quantifying fat accumulation in adipocytes using brightfield microscopy and image analysis.
- This method was validated with the Oil-Red-O staining, a common biochemical method for assessing fat content.
- Their approach, involving thresholding of brightfield images to measure fat droplet areas, allows for quick and accurate analysis of fat accumulation across various cell or tissue types visible under light microscopy.
Article Abstract
A number of biochemical methods are available for measuring fat accumulation in cell culture. The authors report a simple image-based method for measuring fat accumulation in adipocytes using a combination of high-throughput brightfield microscopy and image analysis, which was validated biochemically using Oil-Red-O. The quickest and most accurate method of analysis was one based on thresholding brightfield images and determining the area of fat droplets per image. Thus, the authors have developed a simple high-throughput, label-free method for measuring fat accumulation that is applicable to any cell or tissue type where fat droplets are visible under light microscopy.
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| http://dx.doi.org/10.1177/1087057107306502 | DOI Listing |
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