AI Article Synopsis

  • MLVA, multiplex PCR, and PCR-RFLP were compared for typing Brucella suis isolates and showed perfect agreement in results.
  • MLVA uniquely identified brucellosis outbreaks.
  • It also confirmed that wildlife acts as a reservoir for zoonotic brucellosis.

Article Abstract

Multiple-locus variable-number tandem-repeat analysis (MLVA), multiplex PCR, and PCR-restriction fragment length polymorphism analysis were compared for typing Brucella suis isolates. A perfect concordance was obtained among these molecular assays. However, MLVA was the only method to demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2168551PMC
http://dx.doi.org/10.1128/JCM.01096-07DOI Listing

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