Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1109/emb.2007.901781 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Off-axis electron holography of unstained bacteriophages: Toward electrostatic potential measurement of biological samples.
J Struct Biol
January 2025
CEMES-CNRS, Université de Toulouse, I3EM Team, 29 rue JeanneMarvig B.P, 94347 31055 Toulouse, France. Electronic address:
Transmission electron microscopy, especially at cryogenic temperature, is largely used for studying biological macromolecular complexes. A main difficulty of TEM imaging of biological samples is the weak amplitude contrasts due to electron diffusion on light elements that compose biological organisms. Achieving high-resolution reconstructions implies therefore the acquisition of a huge number of TEM micrographs followed by a time-consuming image analysis.
View Article and Find Full Text PDFLive-cell FRET assay on the stoichiometry and affinity of the YAP complexes in MCF-7 cells.
Arch Biochem Biophys
January 2025
Department of Pain Management, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China. Electronic address:
Yes-associated protein (YAP), a focal point of current biological research, is involved in regulating various life processes. In this report, live-cell fluorescence resonance energy transfer (FRET) imaging was employed to unravel the YAP complexes in MCF-7 cells. Fluorescence imaging of living cells co-expressing CFP (cyan fluorescent protein)-YAP and YFP (yellow fluorescent protein)-LATS1 (large tumor suppressor 1) plasmids revealed that YAP promoted LATS1 oligomerization around mitochondria.
View Article and Find Full Text PDFCDK2 activity crosstalk on the ERK kinase translocation reporter can be resolved computationally.
Cell Syst
January 2025
Department of Biochemistry & BioFrontiers Institute, University of Colorado, Boulder, CO 80303, USA. Electronic address:
The mitogen-activated protein kinase (MAPK) pathway integrates growth factor signaling through extracellular signal-regulated kinase (ERK) to control cell proliferation. To study ERK dynamics, many researchers use an ERK activity kinase translocation reporter (KTR). Our study reveals that this ERK KTR also partially senses cyclin-dependent kinase 2 (CDK2) activity, making it appear as if ERK activity rises as cells progress through the cell cycle.
View Article and Find Full Text PDFImaging features and management of tuberculomas: a review of cases and literature.
Clin Radiol
December 2024
Royal National Orthopaedic Hospital, Brockley Hill, Stanmore, Middlesex HA7 4LP, UK.
Aims: To describe the imaging features of patients presenting with soft tissue masses and a provisional diagnosis of soft tissue tumours, for whom biopsy confirmed the presence of necrotising granulomata consistent with tuberculoma.
Material And Methods: A review of the histopathology database for patients who had a diagnosis of necrotising granulomata in nonspinal sites. Patients with bone and joint-based pathology were excluded.
Single cell derived multicellular meristem: insights into male-to-hermaphrodite conversion and de novo meristem formation in ceratopteris.
Development
January 2025
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA.
Land plants alternate between asexual sporophytes and sexual gametophytes. Unlike seed plants, ferns develop free-living gametophytes. Gametophytes of the model fern Ceratopteris exhibit two sex types: hermaphrodites with pluripotent meristems and males lacking meristems.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!