AI Article Synopsis
- Hydatidosis, caused by the Echinococcus granulosus parasite, poses significant public health and economic challenges as it affects both humans and livestock.
- Calreticulin (CRT), a calcium-binding protein found in most mammalian cells, plays a crucial role in the immune interaction between parasites and their hosts.
- Researchers have cloned segments of EgCRT, proposed a complete cDNA sequence, and found that EgCRT is expressed in both fertile and infertile hydatid cysts, indicating its presence in key cellular areas.
Article Abstract
Hydatidosis, whose etiological agent is the larval stage of the platyhelminth parasite Echinococcus granulosus, is a major zoonotic public health problem and causes great economic losses in many countries, affecting humans and livestock species. Calreticulin (CRT) is a multifunctional and conserved chaperone calcium-binding protein, present in every cell of higher organisms, except erythrocytes. In other parasites species, CRT emerges as a key modulator of several immunological aspects of their relationships with their vertebrate hosts. We report herein the cloning of two DNA segments coding for sequences of E. granulosus CRT (EgCRT). Together with another partial sequence available at the NCBI database, a complete EgCRT cDNA sequence is now proposed. EgCRT mRNA is equally expressed in fertile and infertile hydatid cysts germinal layers, as well as in protoscoleces. In hydatid cysts, EgCRT is a 50 kDa protein, expressed in germinal layer and protoscoleces, mainly in perinuclear and cytoplasmic zones.
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| http://dx.doi.org/10.1016/j.molimm.2007.08.022 | DOI Listing |
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