AI Article Synopsis

  • The study used circular dichroism (CD) and Fourier transform infrared (FTIR) techniques to examine the conformational properties of the alanine-rich peptide Ac-K-[A]11-KGGY-NH2 in different solvents like water, methanol, and trifluoroethanol.
  • The CD spectral analysis showed that the secondary structure of the peptide shifts from alpha-helix to beta-sheet as the peptide concentration increases, with unordered structures being predominant in water.
  • FTIR results indicated that at higher concentrations, solid state beta-sheets dominate in water, while in TFE, alpha-helices and beta-turns are more common; the peptide also demonstrates a tendency to aggregate as shown by the thioflavine T assay

Article Abstract

The circular dichroism (CD) and Fourier transform infrared (FTIR) methods were applied to the conformational studies of alanine-rich peptide Ac-K-[A]11-KGGY-NH2 (where K is lysine, A is alanine, G is glycine and Y is tyrozyne) in water, methanol (MeOH) and trifluoroethanol (TFE). The analysis of CD-spectra of the peptide in water at different concentrations revealed that the secondary structure content depends on the peptide concentration and pH of the solution. The increase of the peptide concentration causes a decrease of alpha-helix content and, simultaneously, an increase of beta-sheet structure, while the unordered structure is the predominant one. Additional elements are discovered in MeOH and TFE but alpha-helix and beta-turns predominate. Moreover, in these solutions the percentage content of the secondary structure does not depend on the temperature. FTIR measurements, carried out at higher peptide concentration (about one order of magnitude) than these CD measurements mentioned above, revealed that in water solution the solid state beta-sheet, and aggregated structures, dominate. However, in TFE the most abundant are alpha-helix and beta-turns structures. The thioflavine T assay showed the tendency of the studied peptide for aggregate.

Download full-text PDF

Source
http://dx.doi.org/10.1002/psc.923DOI Listing

Publication Analysis

Top Keywords

peptide concentration
12
conformational studies
8
studies alanine-rich
8
alanine-rich peptide
8
secondary structure
8
alpha-helix beta-turns
8
peptide
7
peptide ftir
4
ftir spectroscopy
4
spectroscopy circular
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!