Objective: To investigate the flavonoid constituents from aerial parts of Eupatorium odoratum L. systematically.

Methods: The constituents of the EtoAc-soluble portions of the 95% ethanol extraction were isolated and purified by silica gel and sephadex LH-20 chromatography. Their structures were elucidated on the basis of physicochemical properties and spectral analysis.

Results: Eight compounds were isolated and identified as kaempferol-3-methoxy (I), rhamnetin (II), tamarixetin (III), quercetin (IV), kaempferol (V), apigenin (VI), luteolin (VII), dihydrokaempferide (VIII).

Conclusion: Compound I , II and VI were isolated for the first time from genus Eupatorium, and VIII which is rare flavonoid was obtainted for the first time from family Compositae.

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