The improved method for HPLC determination of fatty acids was proposed. The chromatographic separation of p-bromophenacyl derivatives of fatty acids under a gradient elution was achieved at 40 degrees C with an RP-18 LiChroCART 5 column and organic mobile phase containing methanol, acetonitrile, water and TEAP buffer pH 5.6. The quantitative determination of those derivatives was performed at 254 nm. Preeclampsia, the most common pregnancy complication, did not affect triacylglycerol content in the umbilical cord Wharton's jelly in comparison to the control material. However, it changed the composition of fatty acids, bound to that lipid class. The method allows the determination of almost all fatty acids forming the investigated neutral lipid class, contained in a solid tissue sample. The use of TEAP buffer excluded precipitation and flow stoppage in the HPLC system. The method reduced time and costs and might be useful for all other lipid classes and different tissues.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jbbm.2007.09.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Muscle Cell Palmitate-Induced Insulin Resistance, JNK, IKK/NF-κB and STAT3 Activation are Attenuated by Carnosic and Rosmarinic Acid.
Appl Physiol Nutr Metab
January 2025
Brock University, Department of Health Sciences, St Catharines, Ontario, Canada.
The worldwide epidemic of obesity has drastically worsened with the increase in more sedentary lifestyles and increased consumption of fatty foods. Increased blood free fatty acids (FFAs), often observed in obesity, leads to impaired insulin action, and promotes the development of insulin resistance and Type 2 diabetes mellitus (T2DM). JNK, IKK-NF-κB, and STAT3 are known to be involved in skeletal muscle insulin resistance.
View Article and Find Full Text PDFFatty acid composition, total phenolic and total flavonoid contents, and antioxidant activity of Niger seed (Guizotia abyssinica) accessions collected from major producer areas of Ethiopia.
PLoS One
January 2025
Department of Chemistry, College of Natural and Computational Science, Debre Tabor University, Debre Tabor, Ethiopia.
Background: Oils from various sources are vital nutritional components with a variety of roles in our body. Niger seed (Guzoita abyssinica) is endemic to Ethiopia and is among the major oil seed crops grown in the country. The fatty acid composition and the concentration of other bioactive phytochemicals in it vary with species type, geographical origin, cultivation season, and varietal types.
View Article and Find Full Text PDFsp. nov., sp. nov., sp. nov. and gen. nov., sp. nov., isolated from human faeces.
Int J Syst Evol Microbiol
January 2025
Division of Neurogenetics, Center for Neurological Diseases and Cancer, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Small, obligately anaerobic strains 13CB8C, 13CB11C, 13CB18C and 13GAM1G were isolated from a faecal sample in a patient with Parkinson's disease with a history of duodenal resection. After conducting a comprehensive polyphasic taxonomic analysis including genomic analysis, we propose the establishment of one new genus and four new species. The novel bacteria are sp.
View Article and Find Full Text PDFOral Supplements and Photoprotection: A Systematic Review.
J Med Food
January 2025
Integrative Skin Science and Research, Sacramento, California, USA.
Photoprotective effects of various nutritional components and supplements have been demonstrated in animal and studies. The objective of this systematic review is to assess the photoprotective effects of various dietary supplements. A systematic review of studies assessing dietary supplements on photoprotective outcomes was performed.
View Article and Find Full Text PDFCecal microbial composition and serum concentration of short-chain fatty acids in laying hens fed different fiber sources.
Braz J Microbiol
January 2025
Department of Animal Science, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
The intestinal microbiota is widely recognized as an integral factor in host health, metabolism, and immunity. In this study, the impact of dietary fiber sources on the intestinal microbiota and the production of short-chain fatty acids (SCFAs) was evaluated in Lohmann White laying hens. The hens were divided into four treatment groups: a control diet without fiber, a diet with wheat bran (mixed fibers), a diet with insoluble fiber (cellulose), and a diet with soluble fiber (pectin), with six replicates of four hens each.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!