Isolation and sequencing of a PKS gene isolated from xenovulene-producing cultures of Acremonium strictum indicated the presence of NT-, KS-, AT-, PT-, C-MeT- and R-domains; heterologous expression in Aspergillus oryzae resulted in the production of 3-methylorcinaldehyde, demonstrating the role of the terminal reductase domain in product release.
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http://dx.doi.org/10.1039/b708614h | DOI Listing |
Chembiochem
January 2025
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario, M5S 3E5, Canada.
This study describes an enzymatic pathway to produce high purity 4-O-methylglucaric acid from xylan, an underutilized fraction of lignocellulosic biomass. Beechwood xylan was enzymatically hydrolysed using a commercial xylanase and an α-glucuronidase from Amphibacillus xylanus to form 4-O-methylglucuronic acid, which was then purified by anion exchange chromatography and subsequently oxidized to 4-O-methylglucaric acid using a recombinantly produced uronic acid oxidase from Citrus sinensis. Enzymatic oxidation with uronic acid oxidase afforded 95 % yield in 72 hours which is considerably higher than yields previously achieved using a glucooligosaccharide oxidase from Sarocladium strictum.
View Article and Find Full Text PDFMicrob Cell Fact
May 2024
Facultad de Ciencias Exactas y Naturales, Instituto de Ciencias de la Tierra y Ambientales de La Pampa (INCITAP), Universidad Nacional de La Pampa-Consejo Nacional de Investigaciones Científicas y Técnicas (UNLPam-CONICET), Av. Uruguay 151, 6300, Santa Rosa, La Pampa, Argentina.
Background: Low targeting efficacy and high toxicity continue to be challenges in Oncology. A promising strategy is the glycosylation of chemotherapeutic agents to improve their pharmacodynamics and anti-tumoral activity. Herein, we provide evidence of a novel approach using diglycosidases from fungi of the Hypocreales order to obtain novel rutinose-conjugates therapeutic agents with enhanced anti-tumoral capacity.
View Article and Find Full Text PDFPest Manag Sci
July 2024
Laboratorio de Biología Funcional y Biotecnología, BIOLAB-INBIOTEC-CONICET-CICBA. Facultad de Agronomía, UNCPBA, Buenos Aires, Argentina.
Background: Plant defense elicitors are valuable tools in sustainable agriculture, providing an environmentally friendly and effective means of enhancing plant defense and promoting plant health. Fusarium head blight (FHB) is one of the most important fungal diseases of cereal crops worldwide. The PSP1 is a novel biopesticide formulated based on an elicitor, the extracellular protein AsES, from the fungus Sarocladium strictum.
View Article and Find Full Text PDFInsects
November 2023
Department of Entomology, Cornell University, Ithaca, NY 14853, USA.
In the eastern United States, populations of the invasive spotted lanternfly, , are abundant and spreading. Four species of naturally occurring entomopathogenic fungi have previously been reported as infecting these planthoppers, with two of these causing epizootics. Nymphal- and adult-stage lanternflies in Pennsylvania and New York were surveyed for entomopathogenic fungal infections from October 2021 to November 2023, and assays were conducted to confirm the pathogenicity of species that were potentially pathogenic.
View Article and Find Full Text PDFJ Infect
November 2023
Department of General Practice, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China. Electronic address:
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