Graduate School of Science and Technology, Chiba University, Matsudo, Chiba 271-8510, Japan.
Published: November 2007
AI Article Synopsis
Article Abstract
We have developed a restriction landmark genome scanning (RLGS) system in silico, involving two-dimensional electrophoretic analysis of DNA by computer simulation that is based on the availability of whole-genome sequences for specific organisms. We applied the technique to the analysis of the Xanthomonas oryzae pathovar oryzae (Xoo) MAFF 311018, which causes bacterial blight in rice. The coverage that was found to be achievable using RLGS in silico, as a percentage of the genomic regions that could be detected, ranged from 44.5% to 72.7% per image. However, this reached a value of 96.7% using four images that were obtained with different combinations of landmark restriction enzymes. Interestingly, the signal intensity of some of the specific spots obtained was significantly lower than that of other surrounding spots when MboI, which cleaves unmethylated 5'-GATC-3' sites, was used. DNA gel blot analysis with both DNA adenine methylase (Dam)-sensitive and -insensitive isoschizomers (MboI and Sau3AI) revealed that Dam-mediated DNA adenine methylation had indeed occurred at these particular sites. These results suggest that a significant portion of the 5'-GATC-3' sites within the Xoo genome is stably methylated by Dam.
Universidad de la República, Facultad de Ciencias, Instituto de Ciencias Geológicas, Departamento de Paleontología, Iguá, 4225, 11400, Montevideo, Uruguay.
Understanding shell morphology is central to taxonomic and evolutionary studies of bivalves. When traditional systematic studies find limitations in the recognition and classification of taxa, geometric morphometrics methods become a tool to tackle these uncertainties. Taxonomic difficulties are frequently found in systematics studies of the generally homoplastic Pitarinae (Venereidae).
Purpose: We aimed to evaluate the efficacy of moderate doses of bevacizumab in combination with TAS-102 for the treatment of refractory metastatic colorectal cancer.
Methods: A total of 261 patients with refractory mCRC were enrolled and categorized into two groups: TAS-102 combined with bevacizumab and TAS-102 alone. Patients in the bevacizumab combination group were divided into two subgroups based on a median dose of 3.
The superior gluteal nerve (SGN) is a mixed nerve of the sacral plexus that arises from the posterior divisions of the L4, L5, and S1 nerve roots. Its motor branch plays a crucial role in innervation of hip muscles, which allows for physiological gait or walk-pattern. As for its sensory branch, it provides innervation for the hip joint capsule, especially its superior part.
Objective: Despite its potential advantages, robotic surgery has yet to be applied to skull base procedures. Complex anatomy and restricted access have limited the development of robotic skull base surgery. The authors' aim was to conduct a feasibility study of robotic surgery for posterior fossa skull base lesions.
OMFS-IMPATH Research Group, Department of Imaging and Pathology, Faculty of Medicine, KU Leuven, Belgium; Department of Oral and Maxillofacial Surgery, University Hospitals Leuven, Kapucijnenvoer 7, Leuven 3000, Belgium; Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden. Electronic address:
Objectives: To provide an overview of artificial intelligence (AI) applications in orthodontic diagnosis and treatment planning, and to evaluate whether AI improves accuracy, reliability, and time efficiency compared to expert-based manual approaches, while highlighting its current limitations.
Data: This review follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) Checklist.
Sources: An electronic search was performed on PubMed, Web of Science, and Embase electronic databases.
