A flow-injection system for detection of alkaline phosphatase (ALP) activity in human serum samples has been developed. As a specific and inexpensive ALP substrate for this kinetic assay monofluorophosphate (MFP) was applied. For detection of fluoride ions, generated in the course of the biocatalytic hydrolysis of MFP, conventional fluoride ion-selective electrode based on LaF(3)-crystalline membrane was applied. After optimization the system allows analysis of human serum with high selectivity and relatively short time of analysis (5-6 samples h(-1)). Volume of serum required for analysis is 0.05 mL. The system is useful for determination of the enzyme activity in human serum samples at physiological and pathological levels as well as for detection of isoenzymatic forms of ALP.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.aca.2007.02.026 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Does curcumin supplementation affect inflammation, blood count and serum brain-derived neurotropic factor concentration in amateur long-distance runners?
PLoS One
January 2025
Institute of Sport Sciences, Jerzy Kukuczka Academy of Physical Education in Katowice, Katowice, Poland.
Curcumin is known for its potential health benefits; however, the evidence remains inconclusive regarding its necessity as a supplement for athletes during the preparatory phase of training. This study aimed to assess the effect of 6-week curcumin supplementation at a dose of 2g/day on selected inflammatory markers, blood count, and brain-derived neurotropic factor (BDNF) levels in middle-aged amateur long-distance runners during the preparatory period of a macrocycle. Thirty runners were randomly assigned to either a curcumin-supplemented group (CUR, n = 15) or a placebo group (PLA, n = 15).
View Article and Find Full Text PDFA novel combinatorial approach integrating experimental and computational analysis of antioxidant activity: Evaluating catechin and L-ascorbic acid in serum.
PLoS One
January 2025
Chemistry and Biochemistry, University of St. Thomas, Houston, TX, United States of America.
Cardiovascular disease (CVD) remains the leading cause of morbidity and mortality globally, with oxidative stress playing a pivotal role in its progression. Free radicals produced via oxidative stress contribute to lipid peroxidation, leading to subsequent inflammatory responses, which then result in atherosclerosis. Antioxidants inhibit these harmful effects through their reducing ability, thereby preventing oxidative damage.
View Article and Find Full Text PDFVitamin D levels and morbidity and mortality in very low birth weight preterm infants.
Andes Pediatr
August 2023
Departamento de Pediatría, Facultad de Medicina, Universidad de Concepción, Concepción, Chile.
Unlabelled: Very low birth weight (VLBW) preterm newborns lack some nutrients such as vitamin D (VD), which is important in the function and development of different systems.
Objective: To evaluate serum levels of 25-OH-VD in VLBW newborns and to describe the possible association between its deficit and frequent morbidities in this population.
Patients And Methods: Cross-sectional study of VLBW newborns (< 1,500 g and/or < 32 weeks of gestational age).
Sequential intranasal booster triggers class switching from intramuscularly primed IgG to mucosal IgA against SARS-CoV-2.
J Clin Invest
January 2025
Guangzhou Laboratory, Guangzhou International Bio-Island, Guangzhou, China.
The persistent emergence of COVID-19 variants and recurrent waves of infection worldwide underscores the urgent need for vaccines that effectively reduce viral transmission and prevent infections. Current intramuscular (IM) COVID-19 vaccines inadequately protect the upper respiratory mucosa. In response, we have developed a nonadjuvanted, interferon-armed SARS-CoV-2 fusion protein vaccine with IM priming and intranasal (IN) boost sequential immunization.
View Article and Find Full Text PDFIdentification of Butyrylcholinesterase-Derived Small Molecule Peptides Indicative of Novichok Nerve Agent Exposures.
Chem Res Toxicol
January 2025
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, Georgia 30341, United States.
Novichok nerve agents, such as A-230, A-232, and A-234, were classified as Schedule 1 chemicals under the Chemical Weapons Convention (CWC) by the Organisation for the Prohibition of Chemical Weapons (OPCW) following poisoning incidents in 2018. As a result, the production, storage, and use of these chemicals are strictly prohibited by CWC signatory nations. The identification of biomarkers indicating Novichok exposure in humans is crucial for prompt detection and response to potential incidents involving these banned chemical weapons.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!