Micromachined microphones with diffraction-based optical displacement detection have been introduced previously [Hall et al., J. Acoust. Soc. Am. 118, 3000-3009 (2005)]. The approach has the advantage of providing high displacement detection resolution of the microphone diaphragm independent of device size and capacitance-creating an unconstrained design space for the mechanical structure itself. Micromachined microphone structures with 1.5-mm-diam polysilicon diaphragms and monolithically integrated diffraction grating electrodes are presented in this work with backplate architectures that deviate substantially from traditional perforated plate designs. These structures have been designed for broadband frequency response and low thermal mechanical noise levels. Rigorous experimental characterization indicates a diaphragm displacement detection resolution of 20 fm radicalHz and a thermal mechanical induced diaphragm displacement noise density of 60 fm radicalHz, corresponding to an A-weighted sound pressure level detection limit of 24 dB(A) for these structures. Measured thermal mechanical displacement noise spectra are in excellent agreement with simulations based on system parameters derived from dynamic frequency response characterization measurements, which show a diaphragm resonance limited bandwidth of approximately 20 kHz. These designs are substantial improvements over initial prototypes presented previously. The high performance-to-size ratio achievable with this technology is expected to have an impact on a variety of instrumentation and hearing applications.
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http://dx.doi.org/10.1121/1.2769615 | DOI Listing |
Anal Chim Acta
February 2025
School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China. Electronic address:
Background: DNA methylation catalyzed by various DNA methyltransferases (DNA MTases) is one of the important epigenetic regulations in both eukaryotes and prokaryotes. Therefore, the detection of DNA MTase activity is a vital target and direction in the study of methylation-related diseases.
Results: In this study, an ultrasensitive and robust strategy was developed for DNA MTase activity sensing based on bifunctional probe propelling multipath strand displacement amplification and CRISPR/Cas12a techniques.
STAR Protoc
January 2025
Department of Molecular Medicine, University of Pavia, Pavia, Italy. Electronic address:
Voltage-dependent anion channel 1 (VDAC1) is a key protein in cellular metabolism and apoptosis. Here, we present a protocol to express and purify milligram amounts of recombinant VDAC1 in Escherichia coli. We detail steps for a fluorescence polarization-based high-throughput screening assay using NADH displacement, along with procedures for thermostability, fluorescence polarization, and X-ray crystallography.
View Article and Find Full Text PDFSensors (Basel)
December 2024
School of Environment and Resources, Southwest University of Science and Technology, Mianyang 621010, China.
The Daguangbao landslide (DGBL), triggered by the 2008 Wenchuan earthquake, is a rare instance of super-giant landslides globally. The post-earthquake evolution of the DGBL has garnered significant attention in recent years; however, its deformation patterns remain poorly characterized owing to the complex local topography. In this study, we present the first observations of the surface dynamics of DGBL by integrating satellite- and ground-based InSAR data complemented by kinematic interpretation using a LiDAR-derived Digital Surface Model (DSM).
View Article and Find Full Text PDFSensors (Basel)
December 2024
National Key Laboratory of Shock Wave and Detonation Physics, Institute of Fluid Physics, China Academy of Engineering Physics, Mianyang 622150, China.
An ultrafast microwave ranging method based on optically generated frequency-modulated microwave pulses is proposed in this study. The theoretical analysis demonstrated that nanosecond-scale linear frequency modulation microwave pulse can be obtained by femtosecond laser interference under the condition of unbalanced dispersion, which can be used to achieve a high temporal resolution of the displacement change in the measurement by the principle of frequency modulation continuous wave (FMCW) radar. The proof-of-principle experiment successfully measured the displacement change with an error of 2.
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