AI Article Synopsis
- - The study investigates the potential of human dental pulp stem cells (hDPSCs) to differentiate into neuron-like cells under specific conditions, using a cell-clone culture method and an induced medium.
- - hDPSCs were treated with a neural-inductive medium containing various compounds for four days, leading to notable changes in cell morphology and the expression of neuron-specific markers like NSE and GFAP.
- - Results indicate that hDPSCs can differentiate into neuron-like cells upon induction, suggesting their potential for multi-differentiation in regenerative medicine.
Article Abstract
Objective: To explore the multi-differentiated capability of human dental pulp stem cells (hDPSCs) obtained by cell-clone culture approach and to determine the appropriate induced medium.
Methods: The cloned isolation and expansion of hDPSCs were preinduced for 24 h, and were subsequently replaced with neural-inductive medium containing certain concentration of dimethylsulfoxide (DMSO), butylated hydroxyanisode (BHA), forskolin, P-mercaptoethanol (p-ME) and hydrocortisone for 4 days. Then induced cells were analyzed by morphological observation, immnocytochemical staining for non-specific esterase (NSE) and glial fibrillary acidic protein (GFAP) expression, RT-PCR for GFAP mRNA. Meanwhile, the uninduced hDPSCs were used as negative control.
Results: The morphology of induced cells changed at the initial 12 h, and displayed a typical neuron-like cells at 24 h. There was a gradual increase in the number of these neuronal differentiated cells with continuous induction. Furthermore, immnocytochemical staining showed that the induced cell expressed NSE and GFAP, two marked enzymes of neuron cell. The GFAP mRNA was also detected in induced cells by RT-PCR assay. In contrast, the uninduced cells maintained its original appearance and had no expression on them.
Conclusion: hDPSCs may possess potential of multiple-differentiation and may differentiate into neuron-like cells on certain inductive condition.
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