Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice.
Methods: Col2a1-CreER(T2) transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER(T2);R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed.
Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction.
Conclusion: Our findings demonstrate that the Col2a1-CreER(T2) transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2271067 | PMC |
| http://dx.doi.org/10.1016/j.joca.2007.08.001 | DOI Listing |
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