AI Article Synopsis
- The study aimed to evaluate if apoptosis occurs in mouse ovaries after they are vitrified and then warmed.
- The research involved 12-14 day old female mice and utilized ethylene glycol for the vitrification process, assessing follicle viability and ultrastructure through various testing methods.
- Results showed no significant differences in follicle viability or signs of apoptosis between vitrified and non-vitrified ovaries, indicating that vitrification does not induce apoptosis in the ovarian tissues immediately after warming.
Article Abstract
Objective: To evaluate the incidence of apoptosis after vitrification warming of mouse ovaries.
Design: Experimental study.
Setting: University-based research laboratory.
Animal(s): Twelve- to 14-day-old National Medical Research Institute female mice.
Intervention(s): Vitrification of mouse ovaries using ethylene glycol.
Main Outcome Measure(s): Follicle viability assessment by trypan blue testing, morphologic examination by hematoxylin-eosin staining and transmission electron microscopy, apoptosis assessment using the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling method and DNA laddering technique.
Result(s): No statistically significant difference in follicle viability was observed between vitrified and nonvitrified ovaries. On transmission electron microscopy, vitrified ovaries showed a well-preserved ultrastructure. No sign of apoptosis was observed morphologically or by transferase-mediated deoxyuridine triphosphate nick end-labeling technique in either fresh or vitrified-warmed mouse ovaries. Despite the presence of a laddering pattern of DNA in control induced thymic tissue, no similar pattern was observed in fresh or vitrified-warmed ovaries.
Conclusion(s): The data suggest that the vitrification technique does not induce apoptosis in mouse ovarian tissue investigated just after warming.
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| http://dx.doi.org/10.1016/j.fertnstert.2007.07.1384 | DOI Listing |
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