A study on heterogeneity in water-soluble and non-water-soluble protein profiles of different species of Brassica pollen, seeds, and industrial flour for efficient allergy detection and immunotherapy has been lacking in India. The purpose of this study was to examine heterogeneity in the protein profile of antigenic extracts of different species of pollen, seeds, and seed flour of Brassica. A comparison of water-soluble and non-water-soluble protein profiles of seed extracts was studied. Water-soluble and non-water-soluble proteins were extracted, concentration was estimated by Lowry's method, and biochemical characterization by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was analyzed. Pollen from four species, four species of Brassica seeds comprising 25 varieties, and industrial seed flour were included for water-soluble studies, whereas four species of seeds were included for non-water-soluble protein extraction. Significant variation in protein content was observed among four different species of pollen, seeds, and industrial seed flour, respectively. No significant variation was observed in non-water-soluble extracts of four species or among water-soluble and non-water-soluble content of seeds. Heterogeneity in the protein profile of different species of pollen was not observed. However, variation in banding pattern of water-soluble as well as non-water-soluble protein extracts among four different species of seeds was observed. Interestingly, the industrial seed flour also showed a rich protein banding pattern. Variation in protein content as well as protein profile among different species of seeds of Brassica is recorded.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2500/aap.2007.28.3026 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Decay of self-incompatibility within a lifespan in Physalis acutifolia (Solanaceae).
Plant Reprod
January 2025
Department of Ecology and Evolutionary Biology, University of Colorado, 1900 Pleasant Street, Boulder, CO, 80309, USA.
Self-incompatibility decays with age in plants of Physalis acutifolia, and plants that have transitioned to selfing produce fewer seeds but with comparable viability. Self-compatibility in this system is closely related to flower size, which is in turn dependent on the direction of the cross, suggesting parental effects on both morphology and compatibility. The sharpleaf groundcherry, Physalis acutifolia, is polymorphic for self-compatibility, with naturally occurring self-incompatible (SI) and self-compatible (SC) populations.
View Article and Find Full Text PDFSpatially explicit estimation of recent migration rates in plants using genotypic data.
Genetics
January 2025
Institute of Forest Sciences (ICIFOR-INIA), CSIC, Ctra. De la Coruña km 7.5, 28040 Madrid, Spain.
We present a new hierarchical Bayesian method using multilocus genotypes to estimate recent seed and pollen migration rates in a spatially explicit framework that incorporates distance effects separately for each type of dispersal. The method additionally estimates population allelic frequencies, population divergence values, individual inbreeding coefficients, individual maternal and paternal ancestries, and allelic dropout rates. We conduct a numerical simulation analysis that indicates that the method can provide reliable estimates of seed and pollen migration rates and allow accurate inference of spatial effects on migration, at affordable sample sizes (25-50 individuals/population) when population genetic divergence is not low (FST≥0.
View Article and Find Full Text PDFIndian Cryogenebank Conserving Diverse Plant Genetic Resources for the Last Three Decades: Achievements and Way Forward.
Cryobiology
January 2025
ICAR-National Bureau of Plant Genetic Resources, New Delhi-110 012 INDIA.
Ex situ conservation of plant genetic resources (PGR) plays a crucial role in sustainable growth and development, as highlighted by the Global Strategy for Plant Conservation (GSPC). Seed genebanks, a key component of ex situ conservation, have been instrumental in preserving plant diversity. However, challenges arise with the conservation of non-orthodox (recalcitrant and intermediate) seeds and vegetative tissues, which are not amenable to storage in traditional genebanks at temperatures of -20°C.
View Article and Find Full Text PDFOverexpression of Cgo-miR159 Regulates Anther Dehiscence and Pollen Development in and Tobacco.
Genes (Basel)
December 2024
College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China.
Background: MicroRNA159 (miR159) is a conserved miRNA found in various plant species. By regulating GAMYB-like transcription factors, miR159 is involved in diverse biological processes. , a significant traditional Chinese orchid, has unique flower shape and elegant fragrance.
View Article and Find Full Text PDFThe translation initiation factor eIF3M2 upregulates HEAT SHOCK PROTEIN 70 to maintain pollen tube membrane integrity during heat shock.
Plant Physiol
December 2024
Laboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 00 Prague 6, Czech Republic.
Pollen germination and pollen tube (PT) growth are extremely sensitive to high temperatures. During heat stress (HS), global translation shuts down and favors the maintenance of the essential cellular proteome for cell viability and protection against protein misfolding. Here, we demonstrate that under normal conditions, the Arabidopsis (Arabidopsis thaliana) eukaryotic translation initiation factor subunit eif3m1/eif3m2 double mutant exhibits poor pollen germination, loss of PT integrity and an increased rate of aborted seeds.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!