AI Article Synopsis
- Eosinophilic mucus chronic rhinosinusitis (EMCRS) is a severe form of chronic rhinosinusitis (CRS) with frequent recurrences, and the study focuses on the role of the antimicrobial peptide cathelicidin (CAMP) in this condition.
- Biopsy samples were collected from 59 CRS patients and 9 controls to analyze CAMP mRNA and protein levels through various laboratory techniques.
- Results showed higher CAMP mRNA levels in EMCRS patients, indicating its possible protective role, but no significant protein level differences were found among EMCRS, CRS, and control groups.
Article Abstract
Background: Eosinophilic mucus chronic rhinosinusitis (EMCRS) patients are a subgroup of CRS with a poorer prognosis due to frequent recurrences of disease. The cathelicidin antimicrobial peptide (CAMP) is an important innate defense peptide but its role in CRS is not well characterized. The purpose of this study was to investigate CAMP mRNA and protein expression from EMCRS, CRS, and normal control patients.
Methods: Biopsy specimens of nasal mucosa and nasal polyps were taken from 59 CRS patients and 9 controls. CAMP mRNA and protein levels were analyzed by real-time quantitative reverse-transcriptase polymerase chain reaction, immunoassay, Western blot, and immunohistochemistry.
Results: The expression of CAMP mRNA was significantly increased in EMCRS patients compared with CRS patients (p = 0.0004). By immunohistochemistry, expression of CAMP was localized to nasal epithelial, submucosal glands, and inflammatory subepithelial cells. Western blotting confirmed the presence of CAMP in EMCRS, CRS, and control patients. However, we did not detect statistically significant differences in the protein levels in tissue homogenates between EMCRS, CRS, and control patients.
Conclusion: This study shows expression of CAMP in nasal mucosa supporting its role in innate defenses against inhaled pathogens. Although CAMP mRNA was up-regulated in EMCRS patients, there were no statistically significant differences in protein levels in the nasal mucosa of EMCRS compared with CRS patients and controls.
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| http://dx.doi.org/10.2500/ajr.2007.21.3048 | DOI Listing |
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