AI Article Synopsis
- The study aimed to improve gene and antigen detection methods within a plague surveillance program by testing 1798 samples.
- Researchers used various testing methods, including multiple-polymerase chain reaction and enzyme linked immunosorbent assays, alongside traditional culture techniques to identify Yersinia pestis.
- Results showed a positive detection rate of 7.34% with the new methods, which is a significant increase from 6.28%, indicating that the updated approaches may enhance plague diagnosis.
Article Abstract
Objective: To apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.
Methods: 1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.
Results: The overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.
Conclusion: The new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.
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