AI Article Synopsis
- A revertant influenza virus strain was identified that lost temperature-sensitive mutations but retained two specific reversions in the PB2 and PB1 genes.
- Numerous additional mutations (27 in total, with 13 affecting coding sequences) were observed across several viral genes including PB2, PB1, and NP.
- The study suggests that some of these additional mutations might be responsible for suppressing the temperature-sensitive traits in the NS and NP genes.
Article Abstract
A ts+ ca- (non-temperature-sensitive, non-cold-adapted) revertant of the A/Leningrad/134/47/57 ca strain influenza virus [A/Leningrad/134/47/ts+18/1957(H2N2)], obtained in our previous study, lost phenotypic manifestation of ts mutations by the PB2, NP and NS genes, although, according to sequencing data, it acquired only two true reversions of a mutation in the PB2 and PB1 genes. Direct sequencing showed the appearance of 27 additional mutations (13 coding) in the genes encoding the PB2, PB1, PA, NP, M and NS proteins of the revertant, along with the above-mentioned two true reversions. We conjecture that some of these mutations suppressed phenotypic manifestation of ts mutations in the NS and NP genes.
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| http://dx.doi.org/10.1099/vir.0.83014-0 | DOI Listing |
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