A mathematical model of human nucleotide excision repair was constructed and validated. The model incorporates cooperative damage recognition by RPA, XPA, and XPC followed by three kinetic proofreading steps by the TFIIH transcription/repair factor. The model yields results consistent with experimental data regarding excision rates of UV photoproducts by the reconstituted human excision nuclease system as well as the excision of oligonucleotides from undamaged DNA. The model predicts the effect that changes in the initial concentrations of repair factors have on the excision rate of damaged DNA and provides a testable hypothesis on the biochemical mechanism of cooperativity in protein assembly, suggesting experiments to determine if cooperativity in protein assembly results from an increased association rate or a decreased dissociation rate. Finally, a comparison between the random order assembly with kinetic proofreading model and a sequential assembly model is made. This investigation reveals the advantages of the random order assembly/kinetic proofreading model.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702209 | PMC |
| http://dx.doi.org/10.1016/j.jtbi.2007.07.025 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Onabotulinum Toxin A-Led Urinary Tract Infections-Should we Safeguard? A Randomized Controlled Trial.
Int Urogynecol J
January 2025
Department of Urogynecology and Reconstructive Pelvic Surgery, Atlantic Health System, 435 South Street, Suite 370, Morristown, NJ, 07960, USA.
Introduction And Hypothesis: The objective of our study was to evaluate the need for antibiotic prophylaxis for urinary tract infection (UTI) prevention before Onabotulinum toxin A injection for overactive bladder (OAB). We hypothesize that the lack of antibiotic prophylaxis might not be inferior to administering prophylaxis.
Methods: This was a multi-centered, nonblinded, randomized controlled trial conducted between August 2022 and September 2024.
Comparison of postoperative analgesic efficacy between erector spinae plane block and rhomboid intercostal block in breast-conserving surgery and sentinel lymph node biopsy: A randomized non-inferiority clinical trial.
Agri
January 2025
Department of Anesthesiology and Reanimation, İstanbul Medipol University Faculty of Medicine, İstanbul, Türkiye.
Objectives: Breast-conserving surgery is a common breast operation type in the world. Patients may feel severe postoperative pain after the surgery. Several regional anesthesia methods are used for postoperative pain control as a part of multimodal analgesia management after breast surgery.
View Article and Find Full Text PDFThe potential of dynamic plants for attention and stress recovery in indoor environment.
Ergonomics
January 2025
School of Art and Design, Beijing Forestry University, Beijing, China.
This study simulates the natural movement of plants in indoor environments to investigate whether these plants can effectively facilitate psychological, physiological, and emotional recovery from fatigue caused by short vigilance tasks. A total of 63 participants completed baseline assessments of emotional and physiological stress as well as attention and memory (including the POMS-SF, blood pressure, pulse, and Digit Span Backward). They then performed a vigilance task to induce fatigue, followed by a second measurement of stress and cognition.
View Article and Find Full Text PDFThe Role of Negative Affect and Experiential Avoidance in Postsurgical Pain and Fatigue Among Norwegian Women With Breast Cancer.
Eur J Pain
February 2025
Department of Psychology. The Mind-Body Lab, University of Oslo, Oslo, Norway.
Background: Women undergoing breast cancer surgery frequently experience postsurgical pain and fatigue, which reduces their quality of life. Although psychological factors have been shown to play a role in predicting postsurgical outcomes, the subacute recovery phase often remains underexplored. In this secondary analysis of data from a randomised controlled trial, we sought to investigate the predictive role of negative affect and experiential avoidance on postsurgical symptoms during both acute and subacute recovery.
View Article and Find Full Text PDFUsing near-infrared hyperspectral imaging combined with machine learning to predict the components and the origin of Radix Paeoniae Rubra.
Anal Methods
January 2025
College of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.
The efficacy and safety of drugs are closely related to the geographical origin and quality of the raw materials. This study focuses on using near-infrared hyperspectral imaging (NIR-HSI) combined with machine learning algorithms to construct content prediction models and origin identification models to predict the components and origin of Radix Paeoniae Rubra (RPR). These models are quick, non-destructive, and accurate for assessing both component content and origin.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!