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Four-Centre, Multi-Electron Bonding in Rare-Earth Germole Sandwich Complexes.
Angew Chem Int Ed Engl
March 2025
University of Oulu: Oulun Yliopisto, NMR Research Unit, FINLAND.
Reduction of the germole-ligated sandwich complexes [(η5-CpGe)M(η5-Cpttt)]2 (1M, M = Y, Gd, Dy) with one or two equivalents of KC8/2.2.2-cryptand produces [(η5-CpGe)M(η5-Cpttt)2]- (2M) and [(η5-CpGe)M(η5-Cpttt)2]2- (3M), respectively, as salts of [K(2.
View Article and Find Full Text PDFSingle-molecule tracking in living microbial cells.
Biophys Rep
February 2025
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 611731, China.
Some microbes are referred to as model organisms because they are easy to study in the laboratory and hold the ability to retain their characteristics during DNA replication, DNA transcription, and other fundamental processes. Studying these microbes in living cells via single-molecule imaging allows us to better understand these processes at highly improved spatiotemporal resolution. Single particle tracking photoactivated localization microscopy (sptPALM) is a robust tool for detecting the positions and motions of individual molecules with tens of nanometers of spatial and millisecond temporal resolution , providing insights into intricate intracellular environments that traditional ensemble methods cannot.
View Article and Find Full Text PDFIn eukaryotes, the process of intron removal from nuclear pre-mRNA is performed by the spliceosome, a dynamic molecular machine composed of small nuclear ribonucleoproteins (snRNPs; U1, U2, U4, U5, and U6) and dozens of other protein splicing factors. The U6 snRNP contains the U6 snRNA and the proteins Prp24 and Lsm2-8 heteroheptamer. A key feature of the snRNP is a modified U6 snRNA 3' end, which in (yeast) contains a 3' phosphate.
View Article and Find Full Text PDFOn the distance to the transition state of protein folding in optical tweezers experiments.
Biophys Rev
February 2025
Department of Biochemistry and Molecular Biology, Faculty of Chemistry and Pharmaceutical Sciences, Universidad de Chile, Santiago, Chile.
The distance to the transition state ( ) is an important parameter for understanding the energy landscape of chemical reactions. In protein folding, represents the distance to the high energy structure between folded and unfolded states. This correlates with the deformation of the protein as it crosses the energy barrier defining its rigidity.
View Article and Find Full Text PDFA CRISPR/Cas13a system based on a dumbbell-shaped hairpin combined with DNA-PAINT to establish the DCP-platform for highly sensitive detection of Hantaan virus RNA.
Talanta
March 2025
School of Public Health &Jiangxi Provincial Key Laboratory of Disease Prevention and Public Health, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330031, PR China. Electronic address:
Rapid and sensitive detection of specific RNA sequences is crucial for clinical diagnosis, surveillance, and biotechnology applications. Currently, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard for RNA detection; however, it is associated with long processing time, complex procedures, and a high false-positive rate. To address these challenges, we developed a novel sensing platform based on CRISPR/Cas13a that incorporates a dumbbell-shaped hairpin and DNA-PAINT for rapid, highly specific, and sensitive RNA analysis.
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