In hepatocellular carcinoma (HCC), hotspot mutation in codon 249 of the p53 gene has been associated with exposure to aflatoxin B1 (AFB1). While the polymorphism of DNA repair gene X-ray repair cross-complementary group 1 (XRCC1) Arg399Gln may be related with AFB1-DNA adducts and gene mutations. Five hundred one HCCs were included in this study to investigate the role of the XRCC1 codon 399 polymorphism on hotspot mutation in codon 249 of the p53 gene. The genotypes of XRCC1 codon 399 and p53 codon 249 were examined by PCR-RFLP. The HCC patients with XRCC1 genotypes with 399 Gln (namely: XRCC1-AG/GG) exhibited a significantly higher frequency of the p53 hotspot mutations in codon 249 than those with the wild-type homozygote of XRCC1 [namely: XRCC1-AA, adjusted odds ratio (OR) = 6.77, 95% confidence interval (CI) = 4.34-10.57]. Compared with those individuals who did express XRCC1-AA as reference (OR = 1), moreover, individuals featuring XRCC1-AG/GG and AFB1-DNA adducts did experience a significantly greater frequency of the hotspot mutation in codon 249 of the p53 gene (adjusted OR = 28.37, 95% CI = 13.19-61.02, P < 0.01). This study suggests that the XRCC1 Arg399Gln polymorphism and AFB1-DNA adducts are associated with the increased frequency of the p53 mutations in codon 249.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/mc.20384 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Morphometric and phylogenetic analysis of a commercial fish (Teleostei, Leiognathidae).
Zookeys
December 2024
Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University, 201306, Shanghai, China.
The complete mitochondrial genome and phylogenetic analysis for from the South China Sea was performed using shallow genome skimming. For accurate species identification and redescription, morphometric and meristic characters were examined and compared with previous descriptions. To facilitate the identification of species and to enable comparison with the mitochondrial genome phylogeny, molecular comparisons were conducted using three mitochondrial genes: large ribosomal RNA ( rRNA), cytochrome c oxidase subunit 1 (), and NADH dehydrogenase ().
View Article and Find Full Text PDF, and Mutational Profile of Colorectal Cancer in a Series of Moroccan Patients.
Cancer Control
June 2024
Royal School of Military Health Service, Sequencing Unit, Laboratory of Virology, Center of Virology, Infectious, and Tropical Diseases, Mohammed V Military Teaching Hospital, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat, Morocco.
Objectives: The present study aimed to evaluate the frequencies of , and mutations and their possible associations with clinicopathological features in 249 Moroccan patients with colorectal cancer (CRC).
Methods: A retrospective investigation of a cohort of formalin-fixed paraffin-embedded tissues of 249 patients with CRC was screened for // mutations using Idylla™ technology and pyrosequencing.
Results: , and mutations were revealed in 46.
Low Frequency of Aflatoxin Induced TP53 Gene Codon 249 Mutation in Hepatocellular Carcinoma from Egyptian Patients Living in the Nile Delta Region.
Asian Pac J Cancer Prev
September 2023
Department of Pathology, National Liver Institute, Menoufia University, Egypt.
Article Synopsis
- - The study aimed to investigate the presence of a specific mutation (c.747 G>T, p. R249S) linked to Aflatoxin B1 exposure in Egyptian patients diagnosed with hepatocellular carcinoma (HCC).
- - Researchers analyzed 104 tumors for the mutation and assessed TP53 protein expression in 44 of those, finding only 1 out of 104 tumors with the mutation and varied levels of TP53 expression in the samples.
- - Findings indicate a significantly lower occurrence of the TP53 mutation in this sample compared to other studies, implying potential geographical differences in Aflatoxin B1 exposure in Egypt.
Impact of Interaction between Single Nucleotide Polymorphism of XRCC1, XRCC2, XRCC3 with Tumor Suppressor Tp53 Gene Increases Risk of Breast Cancer: A Hospital Based Case-Control Study.
Asian Pac J Cancer Prev
September 2023
Department of Molecular Biology & Genetics, Krishna Vishwa Vidyapeeth (Deemed to be University), Taluka-Karad, Dist- Satara, Pin-415 539, (Maharashtra) India.
Background: At present very little information is available on combined effects of DNA repair genes with tumor suppressor gene polymorphisms and their association with cancer susceptibility. No such association studies have been carried out with breast cancer or any other cancer from India. Present study was conducted to study the combined effects of SNPs of XRCC1, XRCC2, XRCC3 with Arg72Pro and Arg249Ser SNPs of TP53 gene in risk of BC in rural parts of India.
View Article and Find Full Text PDFGene expression and molecular characterization of recombinant subtilisin from Bacillus subtilis with antibacterial, antioxidant and anticancer properties.
Int J Biol Macromol
September 2023
Department of Biosciences and Bioengineering, Indian Institute of Technology Dharwad, Karnataka 580011, India.
This study investigated the multifunctional attributes such as, antibacterial, antioxidant and anticancer potential of recombinant subtilisin. A codon-optimized subtilisin gene was synthesized from Bacillus subtilis and was successfully transformed into E. coli DH5α cells which was further induced for high level expression in E.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!