AI Article Synopsis
- The study focused on analyzing the community structures of anaerobic microflora that produce hydrogen in extreme thermophilic conditions using techniques like DGGE and clone library analyses.
- Researchers enriched extreme thermophilic microflora from cow manure at 75°C with different substrates (xylose, glucose, lactose, etc.) and found varying hydrogen yields for each.
- The analysis revealed that most microflora were closely related to the hydrogen-producing bacterium Caldoanaerobacter subterraneus, with xylose-enriched samples showing greater diversity—including a connection to another thermophile, Caloramator fervidus.
Article Abstract
The present study analyzed the community structures of anaerobic microflora producing hydrogen under extreme thermophilic conditions by two culture-independent methods: denaturing gradient gel electrophoresis (DGGE) and clone library analyses. Extreme thermophilic microflora (ETM) was enriched from cow manure by repeated batch cultures at 75 degrees C, using a substrate of xylose, glucose, lactose, cellobiose, or soluble starch, and produced hydrogen at yields of 0.56, 2.65, 2.17, 2.68, and 1.73 mol/mol-monosaccharide degraded, respectively. The results from the DGGE and clone library analyses were consistent and demonstrated that the community structures of ETM enriched with the four hexose-based substrates (glucose, lactose, cellobiose, and soluble starch) consisted of a single species, closely related to a hydrogen-producing extreme thermophile, Caldoanaerobacter subterraneus, with diversity at subspecies levels. The ETM enriched with xylose was more diverse than those enriched with the other substrates, and contained the bacterium related to C. subterraneus and an unclassified bacterium, distantly related to a xylan-degrading and hydrogen-producing extreme thermophile, Caloramator fervidus.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00253-007-1144-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Variable sediment methane production in response to different source-associated sewer sediment types and hydrological patterns: Role of the sediment microbiome.
Water Res
February 2021
Shanghai Academy of Environmental Sciences, 200233, Shanghai, China. Electronic address:
Article Synopsis
- This study investigates methane production from municipal sewer sediments, highlighting how different types of pollution and water flow patterns affect this process.
- It emphasizes the dominance of acetoclastic methanogenesis in sanitary sewers, driven by specific bacteria interactions, while noting the limited methane production in storm sewers and other contaminated sites.
- The findings stress the importance of hydrology on methane generation, providing insights that could inform strategies for reducing greenhouse gas emissions from sewer systems.
Isolation and culture of Methanobrevibacter smithii by co-culture with hydrogen-producing bacteria on agar plates.
Clin Microbiol Infect
December 2019
Aix-Marseille Université, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France. Electronic address:
Objectives: Methanogenic Archaea are considered as extremely oxygen-sensitive organisms, and their culture is fastidious, requiring specific equipment. We report here conditions allowing the cultivation of Methanobrevibacter smithii in an anaerobic chamber without the addition of hydrogen.
Methods: We first enriched the stool sample in an anaerobic liquid medium.
The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam.
Biotechnol Biofuels
September 2018
1School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo, 454000 China.
Background: Biogenic and biogenic-thermogenic coalbed methane (CBM) are important energy reserves for unconventional natural gas. Thus, to investigate biogenic gas formation mechanisms, a series of fresh coal samples from several representative areas of China were analyzed to detect hydrogen-producing bacteria and methanogens in an in situ coal seam. Complete microbial DNA sequences were extracted from enrichment cultures grown on coal using the Miseq high-throughput sequencing technique to study the diversity of microbial communities.
View Article and Find Full Text PDFElectrochemical Investigations on the Inactivation of the [FeFe] Hydrogenase from Desulfovibrio desulfuricans by O or Light under Hydrogen-Producing Conditions.
Chempluschem
April 2017
Max Planck Institute for Chemical Energy Conversion, Stiftstrasse 34-36, 45470, Mülheim an der Ruhr, Germany.
The applicability of the extremely active [FeFe] hydrogenase from Desulfovibrio desulfuricans in H -producing devices is studied. Despite being the most active enzyme for H catalysis, its high sensitivity towards O has prevented its use in electrolytic water splitting cells. Using electrochemical methods, the catalytic activity of the enzyme at H -producing potentials and its inactivation upon exposure to limited amounts of O or under illumination is analysed.
View Article and Find Full Text PDFEffects of pH and substrate concentrations on dark fermentative biohydrogen production from xylose by extreme thermophilic mixed culture.
World J Microbiol Biotechnol
January 2017
School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, 300384, People's Republic of China.
Biohydrogen is considered as one of the most promising energy alternatives considering the climate and energy crisis. The dark fermentative hydrogen production from xylose at extreme thermophilic condition (70 °C) using mixed culture was conducted in this study. The effects of initial pH values (ranged from 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!