AI Article Synopsis
- The study investigated the effectiveness of three cell lines (HEp-2, NCI-H292, HeLa-I) in isolating Human Respiratory Syncytial Virus (HRSV) from patients with respiratory issues.
- HRSV isolation rates were 46% in HeLa-I, 48% in HEp-2, and 36.3% in NCI-H292, with immunofluorescence being the gold standard, achieving a 53% positive rate.
- Combining all three cell lines improved sensitivity for detecting HRSV to 73.2%, with HeLa-I showing similar effectiveness to HEp-2, suggesting that using multiple cell
Article Abstract
Generally, laboratory diagnosis of viral respiratory infections utilizes virus isolation in cell culture and immunofluorescence assays. In this study, three cell lines (HEp-2, NCI-H292 and HeLa-I) were used for HRSV isolation of strains obtained from patients admitted at HU-USP with respiratory tract disease. HRSV was isolated in 46% (37) of 80 specimens inoculated in HeLa-I, 48% (39) in HEp-2, and 36.3% (29) in NCI-H292. Immunofluorescence was considered the gold standard and yielded 53% positive (43). The results from both methods combined had better sensitivity (73.2%) compared to either method alone. Comparing results between the cell lines with HEp-2 cells as the benchmark, the greatest sensitivity (72.2%) was observed in HeLa-I. This data shows that HeLa-I is adequate for HRSV isolation, giving results similar to the HEp-2 cells. The combined use of the HEp-2, HeLa-I and NCI-H292 cells improve the detection of HRSV.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jviromet.2007.07.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!