Size and shape separation of gold nanoparticles with preparative gel electrophoresis.

J Chromatogr A

Department of Chemistry and Biochemistry and Nanocenter, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, USA.

Published: October 2007

AI Article Synopsis

  • We used agarose gel electrophoresis to separate gold nanoparticles by their size, shape, and charge.
  • We first demonstrated this technique by successfully separating spherical gold nanoclusters of various sizes (5 nm, 15 nm, and 20 nm).
  • Additionally, we purified a crude mixture of gold nanoclusters to obtain a more uniform size and showed that we could also separate different shapes of gold nanoparticles (spheres, plates, and rods).

Article Abstract

We employed agarose gel preparative electrophoresis to separate gold nanoparticles based on size, shape, and charge. The separating technique was first demonstrated by size separation of 5 nm, 15 nm, and 20 nm spherical gold nanoclusters; and further evidenced through the purification of crude 15 +/- 2.7 nm nanoclusters to nanoclusters that were 15 +/- 0.4 nm. The ability to separate gold nanoparticles by shape was also shown by the purification of a mixture of gold spheres, plates, and long rods.

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Source
http://dx.doi.org/10.1016/j.chroma.2007.07.056DOI Listing

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