The aim of this work was to typify the mechanisms involved in gonyautoxins intestinal permeability. For this purpose, permeability of gonyautoxins through intestinal epithelium, and their effect on transepithelial resistance was investigated in excised human jejunal segments. The isolated mucosa segments were mounted in a Ussing chamber and experiments performed under voltage-controlled conditions. Organic gonyautoxin cations were applied in the apical side and samples collected in the basolateral side. Results show that gonyautoxin 2/3 epimers (GTX 2/3) permeate the intestine through a paracellular pathway and, to reach the resolution of the technique we used, no evidence was found of any other transport mechanism involved in the process. A model was developed, according to which tight junctions undergo a toxin concentration and time-dependent change, while transepithelial resistance shows a modest decrease.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2131/jts.32.241 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A rapid LC-MS/MS method for multi-class identification and quantification of cyanotoxins.
Toxicon
October 2023
Laboratory of Toxins and Natural Algae Products, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Environmental Biogeochemistry, Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, Brazil.
Cyanobacteria can form harmful blooms in specific environmental conditions due to certain species producing toxic metabolites known as cyanotoxins. These toxins pose significant risks to public health and the environment, making it critical to identify and quantify them in food and water sources to avoid contamination. However, current screening methods only focus on a single class of cyanotoxins, limiting their effectiveness.
View Article and Find Full Text PDFGPU-based, interactive exploration of large spatiotemporal climate networks.
Chaos
April 2023
Potsdam Institute for Climate Impact Research, Telegrafenberg, 14473 Potsdam, Germany.
This paper introduces the Graphics Processing Unit (GPU)-based tool Geo-Temporal eXplorer (GTX), integrating a set of highly interactive techniques for visual analytics of large geo-referenced complex networks from the climate research domain. The visual exploration of these networks faces a multitude of challenges related to the geo-reference and the size of these networks with up to several million edges and the manifold types of such networks. In this paper, solutions for the interactive visual analysis for several distinct types of large complex networks will be discussed, in particular, time-dependent, multi-scale, and multi-layered ensemble networks.
View Article and Find Full Text PDFDeep learning for x-ray scatter correction in dedicated breast CT.
Med Phys
April 2023
Department of Medical Imaging, Radboud University Medical Center, Nijmegen, The Netherlands.
Background: Accurate correction of x-ray scatter in dedicated breast computed tomography (bCT) imaging may result in improved visual interpretation and is crucial to achieve quantitative accuracy during image reconstruction and analysis.
Purpose: To develop a deep learning (DL) model to correct for x-ray scatter in bCT projection images.
Methods: A total of 115 patient scans acquired with a bCT clinical system were segmented into the major breast tissue types (skin, adipose, and fibroglandular tissue).
Deep Learning Using Endobronchial-Ultrasound-Guided Transbronchial Needle Aspiration Image to Improve the Overall Diagnostic Yield of Sampling Mediastinal Lymphadenopathy.
Diagnostics (Basel)
September 2022
Department of Pathology, Tri-Service General Hospital, Taipei 11490, Taiwan.
Lung cancer is the biggest cause of cancer-related death worldwide. An accurate nodal staging is critical for the determination of treatment strategy for lung cancer patients. Endobronchial-ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) has revolutionized the field of pulmonology and is considered to be extremely sensitive, specific, and secure for lung cancer staging through rapid on-site evaluation (ROSE), but manual visual inspection on the entire slide of EBUS smears is challenging, time consuming, and worse, subjective, on a large interobserver scale.
View Article and Find Full Text PDFGonyautoxins 2/3 Local Periarticular Injection for Pain Management after Total Knee Arthroplasty: A Double-Blind, Randomized Study.
J Knee Surg
March 2023
Membrane Biochemistry Laboratory, Department of Physiology and Biophysics, Faculty of Medicine Universidad de Chile, Santiago, Chile.
The purpose of this study was to compare the efficacy of periarticular infiltration of gonyautoxin 2/3 (GTX 2/3) and a mixture of levobupivacaine, ketorolac, and epinephrine for pain management after total knee arthroplasty (TKA). Forty-eight patients were randomly allocated to receive periarticular infiltration of 40 µg GTX 2/3 ( = 24) diluted in 30 mL of sodium chloride 0.9% (study group) or a combination of 300 mg of levobupivacaine, 1 mg of epinephrine, and 60 mg ketorolac ( = 24) diluted in 150 mL of sodium chloride 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!