AI Article Synopsis
- IGF-I is crucial for regulating pituitary and gonadal functions, which are important for sexual development and fertility in mammals.
- Using a specific method to inactivate the IGF-I receptor in Sertoli cells, researchers found that the absence of this receptor leads to fewer viable Sertoli cells due to reduced cell growth and increased cell death.
- The loss of IGF-IR also changes the shape of Sertoli cells and lowers their secretion of important substances like lactate and transferrin, indicating that autocrine IGF-I plays a key role in maintaining Sertoli cell health.
Article Abstract
IGF-I regulates pituitary and gonadal functions, and is pivotal for sexual development and fertility in mammalian species. To better understand the function of autocrine IGF-I in Sertoli cell physiology, we established a system for Cre-mediated conditional inactivation of the IGF-I receptor (IGF-IR) in cultured Sertoli cells. We show here that loss of IGF-IR decreased the number of viable Sertoli cells as a consequence of diminished Sertoli cell proliferation and increased Sertoli cell death. Furthermore, the lack of IGF-IR altered the morphology of cultured Sertoli cells and decreased lactate and transferrin secretions. Collectively, our data indicate that autocrine IGF-I contributes significantly to Sertoli cell homeostasis. The described in vitro system for loss-of-function analysis of the IGF-IR can be readily transposed to study the role of other intratesticular growth factors involved in spermatogenesis.
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| http://dx.doi.org/10.1677/JOE-07-0258 | DOI Listing |
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