AI Article Synopsis
- An interlaboratory study assessed a method for detecting aristolochic acid I in plant species and dietary supplements, using aqueous acetonitrile for extraction and liquid chromatography (LC) with UV detection for analysis.
- The study involved 10 collaborators who successfully analyzed 13 blind duplicates, confirming the presence of aristolochic acid I in one sample by 8 labs, indicating reliable testing across different labs.
- Results showed acceptable repeatability and reproducibility, with relative standard deviations ranging from 1.72% to 19.8%, while calibration curves for all labs had high correlation coefficients above 0.998, ensuring method reliability.
Article Abstract
An interlaboratory study was conducted to evaluate a method for the determination of aristolochic acid I, also known as aristolochic acid A, at levels > 2.00 microg/g in botanical species and dietary supplements potentially contaminated with aristolochic acid I. Aristolochic acid I was extracted from various matrixes with aqueous acetonitrile. The amount of aristolochic acid I present was determined by liquid chromatography (LC) using an ultraviolet (UV) detector with confirmation by LC/mass spectrometry (MS). Thirteen blind duplicates were successfully analyzed by 10 collaborators, and aristolochic acid I was successfully confirmed in 1 blind duplicate by 8 collaborators. For repeatability, the relative standard deviation (RSD(r)) ranged from 1.72 to 16.3% and for reproducibility, the RSDR ranged from 5.42 to 19.8%. HorRat values were not applicable for 2 materials but varied from 0.7 to 1.8 for 11 materials. Each collaborating laboratory had calibration curves with correlation coefficients > 0.998. In addition, all of the collaborators that conducted the confirmation were able to verify the identity of aristolochic acid I using LC/MS/MS (using either ion trap or triple quad).
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