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Culture media design and scaling-up of submerged fermentation for the nematophagous fungus Duddingtonia flagrans.
Exp Parasitol
January 2025
Corporación colombiana de investigación agropecuaria - AGROSAVIA, Bioproducts Department, Mosquera - Colombia.
Biological control, which utilizes nematophagous fungi to reduce gastrointestinal nematode populations, may effectively diminish the need for chemical anthelmintic treatments. However, the limited knowledge surrounding the mass production of chlamydospores hinders the widespread use of biological products as alternatives to traditional anthelmintics. This study aimed to evaluate the development of liquid culture media for the large-scale production of the nematophagous fungi Duddingtonia flagrans using a systematic procedure, progressing from microplates to bioreactor.
View Article and Find Full Text PDFCytotoxicity evaluation of microbial sophorolipids and glucolipids using normal human dermal fibroblasts (NHDF) .
Toxicol Rep
June 2025
Sorbonne Université, Centre National de la Recherche Scientifique, Laboratoire de Chimie de la Matière Condensée de Paris, LCMCP, Paris F-75005, France.
Unlabelled: Fibroblasts are considered a key player in the wound healing process. Although this cellular family is constituted by several distinct subtypes, dermal fibroblasts are crucial thanks to their ability to secrete pro-regenerative growth factors, extracellular matrix (ECM) proteins and their immune and anti-inflammatory role. Sophorolipids (SL), sophorosides (SS) and glucolipids (G), mono-unsaturated (C18:1) or saturated (C18:0), glycolipids derived from microbial fermentation of wild type or engineered yeast , constitute a novel sustainable class of bio-based chemicals with interesting physicochemical characteristics, which allow them to form soft diverse structures from hydrogels to vesicles, micelles or complex coacervates with potential interest in skin regeneration applications.
View Article and Find Full Text PDFEffects of ORF14 gene on melanin expression, fermentation conditions and properties of melanin production in modified strains.
J Biotechnol
January 2025
Key Laboratory of Green Manufacturing and Biosynthesis of Food Bioactive Substances, China General Chamber of Commerce, School of Food and Health, Beijing Technology and Business University, Beijing, 100048 China.
Melanin with antioxidant and antibacterial properties can be used in food, cosmetics, biotechnology, and other fields, but its insolubility become a main challenge hindering for its application. In this study, water-soluble melanin produced by the novel species Streptomyces vilmorinianum YP1 was characterized using scanning electron microscopy (SEM), UVvisible spectroscopy (with an absorption peak at 220nm), and Fourier transform infrared (FTIR) spectroscopy. The glycosyltransferase gene ORF14 was knocked out, which improved the production of water-soluble melanin by inhibiting competitive pathway.
View Article and Find Full Text PDFComparison of Streptococcus mutans biofilm formation, acidogenicity, and buffering capacity among human breast milk, plain packaged bovine milk, sweetened bovine milk, and infant formula: An in vitro study.
J Indian Soc Pedod Prev Dent
October 2024
Department of Pedodontics and Preventive Dentistry, Sardar Patel Post Graduate Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India.
Article Synopsis
- Human milk (HBM) and bovine milk (PBM) are both sources of nutrition that involve lactose, which can be fermented by the bacteria Streptococcus mutans, potentially affecting dental health.
- This study compares how S. mutans forms biofilms, produces acid, and buffers in HBM, plain and sweetened PBM, and infant formula (IF) through various microbiological assays.
- Results indicated that sweetened bovine milk had the highest biofilm formation and lowest pH, while both HBM and PBM showed low cariogenicity, differing from the effects of sucrose.
Effectiveness of the SYNCHRONIZE + Brief Intervention in Improving Mediterranean Diet Adherence, Nutritional Quality and Intake Pattern in Persons with Fibromyalgia and Chronic Fatigue Syndrome.
Nutrients
December 2024
Unitat de Suport a la Recerca Terres de l'Ebre, Fundació Institut Universitari per a la Recerca a l'Atenció Primària de Salut Jordi Gol I Gurina (IDIAPJGol), 43500 Tortosa, Spain.
Background: Multidisciplinary lifestyle interventions are being researched to treat fibromyalgia. However, the impact of nutrition as a key treatment component is little studied. This study aimed to evaluate the effectiveness of the SYNCHRONIZE + lifestyle multidisciplinary intervention in improving adherence to the Mediterranean diet, nutrition quality and dietary intake pattern in persons with fibromyalgia and chronic fatigue syndrome.
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