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Transcriptomic Changes in Human Tonsil-Derived Mesenchymal Stem Cells Across Culture Passages.
Genes (Basel)
December 2024
Department of Biochemistry and Molecular Biology, Kangwon National University School of Medicine, Chuncheon 24341, Republic of Korea.
Background/objectives: Tonsil-derived mesenchymal stem cells (TMSCs) are in the limelight in regenerative medicine due to their high proliferation and differentiation potential. It is important to conduct studies to determine the optimal conditions for achieving the maximum yield while maintaining the optimal differentiation capacity of TMSCs.
Methods: This study explores the impact of serial subculture on TMSCs by analyzing gene expression at passages 2, 4, 6, and 8.
Antibiotic susceptibility testing and molecular characterization based on whole-genome sequencing of isolates from pediatric infections at the National Regional Medical Center of Southwest China during the COVID-19 pandemic.
Front Public Health
December 2024
Department of Laboratory Medicine, West China Second University Hospital, Sichuan University, Chengdu, China.
Background: is a transmitted respiratory pathogen that causes high morbidity and mortality in children, especially those under 5 years of age. During the implementation of population control measures for COVID-19 in mainland China, the detection rate in pediatric patients decreased. However, with the second wave of the COVID-19 pandemic (2022), the incidence of pneumococcal disease (PD) and even invasive pneumococcal disease (IPD) began to rise again.
View Article and Find Full Text PDFExploring the Genetic Landscape of Mycobacterium tuberculosis: Unlocking the Differences in Between Latent and Active Tuberculosis.
Int J Mycobacteriol
October 2024
Department of Global Public Health, Karolinska Institute, Stockholm, Sweden.
Background: Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), can enter a dormant phase within host tissues, complicating treatment and highlighting the need to investigate the genetic changes associated with dormancy.
Methods: This study examined clinical isolates of MTB, representing a range of susceptibility profiles and standard reference laboratory strains, i.e.
In vitro Genebank of India for safe conservation of horticultural plant diversity: four decades of milestones.
3 Biotech
January 2025
ICAR- National Bureau of Plant Genetic Resources, New Delhi, 110012 India.
India is a treasure trove of biological diversity with its plant genetic resources playing a crucial role in the crop improvement serving as the foundation for the country's sustainable food and nutritional security. India's in vitro genebank (IVG) is part of the National Genebank at the Indian Council of Agricultural Research-National Bureau of Plant Genetic Resources (ICAR-NBPGR). The IVG houses distinctive multi-crop repository that utilizes several tissue culture techniques for short- to medium-term storage in in vitro active genebank (IVAG) and cryoconservation approaches for long-term storage in in vitro base genebank (IVBG).
View Article and Find Full Text PDFBackground: Differentiation of patient-specific induced pluripotent stem cells (iPS) helps researchers to study the individual sensibility to drugs. However, differentiation protocols are time-consuming, and not all tissues have been studied. Few works are available regarding pancreatic exocrine differentiation of iPS cells, and little is known on culturing and cryopreserving these cells.
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