AI Article Synopsis
- Microsatellites are crucial genetic markers used to study species that haven't been thoroughly researched, and they are typically isolated using enriched genomic libraries.
- In a study comparing two insect species, the ant Lasius austriacus and the beetle Pityogenes chalcographus, researchers found significant differences in the number of microsatellite loci successfully isolated.
- The challenges faced while isolating markers in the beetle, such as repeated identical motifs and problems with primer development, highlighted important steps in the microsatellite marker development process.
Article Abstract
Microsatellites are powerful markers often isolated de novo for species yet to be investigated. Enriched genomic libraries are usually used for isolation purposes. We critically evaluate the outcome of an enrichment-based protocol applied to two insect species (the ant Lasius austriacus and the beetle Pityogenes chalcographus) which yielded contrasting numbers of suitable loci. Our findings of differences in microsatellite isolation are consistent with the available data on differences in genomic characteristics across these taxa. In the beetle repeated isolation of identical motifs, difficulties in primer development, and multibanded products caused loss of most candidate clones. We identified critical steps during marker development.
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| http://dx.doi.org/10.1007/s00239-007-9012-1 | DOI Listing |
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