Biochemical identification of mycobacteria is slow and many times fail to produce correct results. We compared PCR-restriction fragment length polymorphism analysis (PRA) of hsp65 and biochemical methods for the identification of mycobacteria from human samples in Belgium. PRA was found useful in the identification of mycobacteria and simple to implement as a quick method in the laboratory.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mimet.2007.07.008DOI Listing

Publication Analysis

Top Keywords

identification mycobacteria
16
samples belgium
8
application hsp65
4
hsp65 pra
4
pra method
4
method rapid
4
identification
4
rapid identification
4
mycobacteria
4
mycobacteria isolated
4

Similar Publications

CRISPR-Cas12a-based detection and differentiation of Mycobacterium spp.

Clin Chim Acta

December 2024

Center of Excellence in Systems Microbiology (CESM), Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Rama IV, Bangkok 10330, Thailand. Electronic address:

Mycobacterium species cause several vital human diseases, including tuberculosis and non-tuberculous mycobacterial infections which are treated with different drug regimens Therefore, accurate and rapid diagnosis is essential for effective treatment and controlling the spread of these pathogens. This study aims to develop the isothermal method combining RPA and CRISPR-Cas12a techniques, named as MyTRACK, to detect and differentiate major clinical mycobacteria at the species level. The assay has no cross-reactivity with limit of detection of 1 to 100 copies/reaction for various targeted mycobacteria.

View Article and Find Full Text PDF

Recent advances in nanomaterials for the detection of mycobacterium tuberculosis (Review).

Int J Mol Med

March 2025

Orthopedics of Chun'an First People's Hospital, Zhejiang Provincial People's Hospital Chun'an Branch, Affiliated Chun'an Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 311700, P.R. China.

The world's leading infectious disease killer tuberculosis (TB) has >10 million new cases and ~1.5 million mortalities yearly. Effective TB control and management depends on accurate and timely diagnosis to improve treatment, curb transmission and reduce the burden on the medical system.

View Article and Find Full Text PDF

The value of metagenomic next-generation sequencing with blood samples for the diagnosis of disseminated tuberculosis.

Front Cell Infect Microbiol

December 2024

Department of Infectious Diseases, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Objective: The aim of this study was to assess the clinical value of metagenomic next-generation sequencing (mNGS) of blood samples for the identification of disseminated tuberculosis (DTB).

Methods: A total of 48 individuals suspected of DTB were enrolled. All patients underwent mNGS of peripheral blood and conventional microbiological tests.

View Article and Find Full Text PDF

Mycobacterium tuberculosis (Mtb) remains a leading infectious disease responsible for millions of deaths. RNA sequencing is a rapidly growing technique and a powerful approach to understanding host and pathogen cross-talks via transcriptional responses. However, its application is limited due to the high costs involved.

View Article and Find Full Text PDF

The underlying mechanisms used by the intestinal microbiota to shape disease outcomes of the host are poorly understood. Here, we show that the gut commensal protozoan, Tritrichomonas musculis (T.mu), remotely shapes the lung immune landscape to facilitate perivascular shielding of the airways by eosinophils.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!