AI Article Synopsis
- Bone resorption by osteoclasts encourages the formation of new bone by osteoblasts, and researchers investigated the genes involved in this process using microarray analysis.
- They identified osteomodulin (OMD) as a key factor that increases alongside osteoclast-specific markers, showing its expression in osteoblasts during their maturation.
- In experiments with op/op mice, which lack functional osteoclasts, OMD levels were lower, but the expression could be increased with M-CSF treatment, suggesting OMD is likely a marker for osteoblast maturation influenced by osteoclast activity.
Article Abstract
Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglycan, was simultaneously induced with osteoclast-specific markers such as MMP9 and Acp5. OMD expression was detected in osteoblasts and upregulated during osteoblast maturation. OMD expression in osteoblasts was also detected immunohistochemically using a specific antibody against OMD. The immunoreactivity against OMD decreased in op/op mice, which lack functional macrophage colony stimulating factor (M-CSF) and are therefore defective in osteoclast formation, when compared to wild-type littermates. OMD expression in op/op mice was upregulated by M-CSF treatment. Since the M-CSF receptor c-Fms was not expressed in osteoblasts, it is likely that OMD is an osteoblast maturation marker that is induced by osteoclast activity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2007.07.193 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification and validation of diagnostic biomarkers and immune infiltration in dilated cardiomyopathies with heart failure and construction of diagnostic model.
Gene
January 2025
Department of Cardiology, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, 450000, China; Heart Center of Henan Provincial People's Hospital, Central China Fuwai Hospital, Central China Fuwai Hospital of Zhengzhou University, Zhengzhou, 450000, China. Electronic address:
Article Synopsis
- Dilated cardiomyopathy (DCM) can lead to heart failure and is marked by immune cell infiltration; researchers conducted extensive analysis on various datasets to identify key genes and biomarkers related to this condition.
- Using tools like CytoHubba and ssGSEA, the study pinpointed important diagnostic biomarkers (OMD and THBS4) and revealed their connections to immune cells, while also predicting transcription factors, microRNAs, and potential drug candidates.
- Validation in a mouse model confirmed that these biomarkers correlate with gene expression trends found in earlier bioinformatic analyses, offering new insights for diagnosing and treating DCM with heart failure.
Identification of Potential Clusters of Signs and Symptoms to Prioritize Patients' Eligibility for AADCd Screening by 3-OMD Testing: An Italian Delphi Consensus.
Behav Neurol
September 2024
Child Neurology and Psychiatry Unit, AUSL-IRCCS di Reggio Emilia, Reggio Emilia, Italy.
Introduction: AADCd is an ultrarare, underdiagnosed neurometabolic disorder for which a screening test (3-OMD dosing on dried blood spot (DBS)) and targeted gene therapy (authorized in the EU and the UK) are available. Therefore, it is mandatory to raise awareness of presenting symptoms and signs among practitioners. Delivering scientifically sound information to promote screening of patients with the correct cluster of symptoms and signs would be critical.
View Article and Find Full Text PDFTranscriptomic landscape of quiescent and proliferating human corneal stromal fibroblasts.
Exp Eye Res
November 2024
Harry S. Truman Memorial Veterans' Hospital, Columbia, MO, USA; Department of Veterinary Medicine & Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA; Department of Ophthalmology, School of Medicine, University of Missouri, Columbia, MO, USA. Electronic address:
This study analyzed the transcriptional changes in primary human corneal stromal fibroblasts (hCSFs) grown under quiescent (serum-free) and proliferating (serum-supplemented) culture conditions to identify genes, pathways, and protein‒protein interaction networks influencing corneal repair and regeneration. Primary hCSFs were isolated from donor human corneas and maintained in serum-free or serum-laden conditions. RNA was extracted from confluent cultures using Qiagen kit and subjected to RNA sequencing (RNAseq) analysis.
View Article and Find Full Text PDFVaried clinical presentations of RP1L1 variants in Chinese patients: a study of occult macular dystrophy and vitelliform macular dystrophy.
BMC Ophthalmol
August 2024
Department of Ophthalmology, Southwest Hospital of Army Medical University, Chongqing, 400038, China.
Background: Occult Macular Dystrophy (OMD), primarily caused by retinitis pigmentosa 1-like 1 (RP1L1) variants, is a complex retinal disease characterised by progressive vision loss and a normal fundus appearance. This study aims to investigate the diverse phenotypic expressions and genotypic correlations of OMD in Chinese patients, including a rare case of Vitelliform Macular Dystrophy (VMD) associated with RP1L1.
Methods: We analysed seven OMD patients and one VMD patient, all with heterozygous pathogenic RP1L1 variants.
Neuroprotective effect of omidenepag on excitotoxic retinal ganglion cell death regulating COX-2-EP2-cAMP-PKA/Epac pathway via Neuron-Glia interaction.
Neuroscience
August 2024
Department of Ophthalmology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Article Synopsis
- Glutamate excitotoxicity contributes to the death of retinal ganglion cells (RGCs) in conditions like glaucoma and ischemia-reperfusion injury, causing both direct and indirect damage through inflammation.
- The study focused on Omidenepag (OMD), a new drug that lowers intraocular pressure and acts as an EP2 receptor agonist, investigating its potential neuroprotective effects against excitotoxic RGC death.
- OMD was found to significantly reduce RGC death and inflammatory responses in both in vitro and in vivo models, suggesting it promotes protective pathways while inhibiting harmful ones by improving interactions between glial cells and neurons.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!