Dietary restriction does not alter retinal aging in the Fischer 344 rat.

In the present study, the effects of long-term dietary restriction (60% of the calories in the ad lib diet, beginning at 16 weeks of age) on quantitative morphometric measures and histopathologic indications of aging have been investigated in the retina of Fischer 344 male rats. The animals were maintained by the NIA Biomarkers Program, National Center for Toxicological Research. Group size ranged from 8 to 15 rats. A gradual thinning of the outer nuclear layer (ONL) of photoreceptor nuclei occurred with aging in control ad lib groups. The restricted diet did not affect retinal aging in 18-, 21-, 26-, or 27-month-old rats, as judged by photoreceptor cell death, ONL thickness, and pattern of cell loss. Retinal thickness (RT) was unaffected by restricted diet, except in the 21-month-old group; in that group, the RT was reduced significantly in thickness as compared to ad lib animals. These results are in contrast to studies of dietary restriction on most nonneuronal markers of aging and suggest that a different mechanism may modulate at least some aspects of brain aging.