AI Article Synopsis
- The study investigates how nanosecond pulsed electric fields (nsPEFs) affect the electrical properties of Jurkat cells, a type of human T-cell.
- Using time domain dielectric spectroscopy, researchers observed significant changes in the conductivity of the cells' cytoplasm and nucleoplasm after exposure to electric pulses.
- The results indicate that electropermeabilization likely occurred, allowing ions to move out of the cells, and suggest potential long-term damage to cell membranes from repeated exposure to these electrical pulses.
Article Abstract
The interaction of nanosecond duration pulsed electric fields (nsPEFs) with biological cells, and the models describing this behavior, depend critically on the electrical properties of the cells being pulsed. Here, we used time domain dielectric spectroscopy to measure the dielectric properties of Jurkat cells, a malignant human T-cell line, before and after exposure to five 10ns, 150kV/cm electrical pulses. The cytoplasm and nucleoplasm conductivities decreased dramatically following pulsing, corresponding to previously observed rises in cell suspension conductivity. This suggests that electropermeabilization occurred, resulting in ion transport from the cell's interior to the exterior. A delayed decrease in cell membrane conductivity after the nsPEFs possibly suggests long-term ion channel damage or use dependence due to repeated membrane charging and discharging. This data could be used in models describing the phenomena at work.
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| http://dx.doi.org/10.1016/j.bbrc.2007.07.159 | DOI Listing |
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