AI Article Synopsis
- Researchers established an in vitro immunization protocol using human PBMCs treated with LLME and sensitized with antigens and interleukins to produce antigen-specific antibodies.
- The study investigated the immunomodulatory effects of Propionibacterium acnes (P. acnes) on these PBMCs, as P. acnes has shown various effects in mouse models.
- Results indicated that P. acnes enhances the production of antigen-specific antibodies by possibly boosting inflammatory cytokine levels and T-B cell interactions, suggesting it may serve as an effective adjuvant in antibody responses during in vitro immunization.
Article Abstract
We have established an in vitro immunization protocol whereby human peripheral blood mononuclear cells (PBMCs) are initially treated with L-leucyl-L-leucine methyl ester (LLME) and subsequently sensitized with antigen in the presence of interleukin (IL)-2, IL-4, and adjuvant. This protocol resulted in the production of antigen-specific antibodies. PBMCs are potentiated to react with exogenous antigens upon treatment with LLME. We are using this system to investigate the immunomodulatory activity of additives. In the present study, we aimed to evaluate the immunomodulatory activity of Propionibacterium acnes (P. acnes), which is known to exhibit various immunomodulatory effects in murine models, using this in vitro immunization protocol. P. acnes was found to augment the production of antigen-specific antibodies by PBMC, possibly through increased production of inflammatory cytokines and/or increased T-B cell interaction. P. acnes hence appears to act as an adjuvant in the antibody response in in vitro immunization.
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| http://dx.doi.org/10.1271/bbb.70159 | DOI Listing |
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