AI Article Synopsis
- The study utilized high performance liquid chromatography (HPLC) to analyze a 5kDa component from enamel matrix derivative (EMD), which is used in periodontal regeneration.
- Initial purification was done with size-exclusion chromatography (SEC), and subsequent analysis revealed four distinct peaks of the 5kDa component through reversed-phase HPLC (RP HPLC).
- From an initial 1200 mg of EMD, about 65 mg of the 5kDa component was obtained, achieving a 60% recovery rate, with SEC being suitable for qualitative and RP HPLC suitable for both qualitative and quantitative analysis.
Article Abstract
High performance liquid chromatography (HPLC) methods were used to analyse a 5kDa component purified from enamel matrix derivative (EMD), the active ingredient in Emdogain, a commercial product for periodontal tissue regeneration. After initial purification by size-exclusion chromatography (SEC) on a 100 cm x 5 cm column (Bio-Gel P-30 Fine, 280 nm), collected fractions were analysed by size-exclusion HPLC (SE HPLC; TSK-Gel Super SW2000, 220 nm). The fractions containing only the 5kDa component were analysed by reversed-phase high-pressure chromatography (RP HPLC; YMC-Pack ODS-A, 200 nm), revealing four peaks of the 5kDa component. From 1200 mg of EMD (of which 9% is the 5kDa component), approximately 65 mg of lyophilised 5kDa component were obtained, corresponding to a recovery of 60%. The SE HPLC method was mainly suitable for qualitative analysis, whereas the RP HPLC method was appropriate for both qualitative and quantitative analysis.
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| http://dx.doi.org/10.1016/j.jchromb.2007.07.017 | DOI Listing |
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