Background: Siglec-7, a sialic acid binding inhibitory receptor expressed by NK cells is masked in vivo by a so far unknown ligand. It shows a strong binding prevalence for alpha-2,8-linked disialic acids in vitro.
Results: Here we describe the expression of PSA-NCAM (alpha-2,8-linked polysialic acid modified NCAM) on functional adult peripheral blood natural killer cells and examine its possible role in masking Siglec-7. Unmasking of Siglec-7 using Clostridium perfringens neuraminidase massively reduces NK cell cytotoxicity. By contrast a specific removal of PSA using Endo-NF does not lead to a reduction of NK cell cytotoxicity.
Conclusion: The results presented here therefore indicate that PSA-NCAM is not involved in masking Siglec-7.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1976417 | PMC |
| http://dx.doi.org/10.1186/1471-2172-8-13 | DOI Listing |
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