Objective: To investigate the effect of thaspine on the cellular proliferation, apoptosis and cell cycle in A549 cell line.
Methods: A549 cell was cultured with different concentrations of thaspine. Cellular proliferation was detected with MTT, apoptosis and cell cycle were checked with Flow Cytometer, and change of microstructure was observed by transmission electron microscope.
Results: Thaspine could inhibit the proliferation and induce apoptosis of A549 cell in a time-dose dependent manner. Cell cycle was significantly stopped at the S phase by thaspine with FCM technology. Under electronic microscope, the morphology of A549 cell showed nuclear karyopycnosis, chromatin agglutination and typical apoptotic body when the cell was treated with thaspine.
Conclusion: Thaspine has the effects of anti-tumor and inducing apoptosis.
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