The micro-colony method was used to enumerate viable bacteria in composts. Cells were vacuum-filtered onto polycarbonate filters and incubated for 18 h on LB medium at 37 degrees C. Bacteria on the filters were stained with SYBR Green II, and enumerated using a newly developed micro-colony auto counting system which can automatically count micro-colonies on half the area of the filter within 90 s. A large number of bacteria in samples retained physiological activity and formed micro-colonies within 18 h, whereas most could not form large colonies on conventional media within 1 week. The results showed that this convenient technique can enumerate viable bacteria in compost rapidly for its efficient quality control.
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http://dx.doi.org/10.1016/j.mimet.2007.06.019 | DOI Listing |
J Indian Soc Pedod Prev Dent
October 2024
Department of Pediatric and Preventive Dentistry, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.
Background And Objectives: The purpose of this study was to evaluate the antimicrobial activity of methylene blue dye with 660-nm diode laser in cavity disinfection and to compare the total bacterial count in dentinal samples preexcavation, postexcavation, and postdisinfection. The study design was experimental in vivo.
Materials And Methods: Fifteen children aged 5-12 years with 15 primary molars requiring atraumatic restorative treatment (ART) were selected.
Virol J
January 2025
Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague, Czech Republic.
Hepatitis B virus (HBV) infection can cause liver disease and lead to hepatocellular carcinoma (HCC). To better understand the factors involved in viral infection and pathogenesis and to develop novel therapies, it is crucial to investigate virus-host interactions. HBV infection has been shown to increase the expression of the unconventional prefoldin RPB5 interactor (URI1), a cellular protein that promotes liver tumorigenesis and HCC metastasis.
View Article and Find Full Text PDFClin Microbiol Infect
January 2025
Chlamydia Group, Institute of Veterinary Pathology; University of Zürich, Switzerland.
Objectives: Chlamydia trachomatis is the most commonly diagnosed bacterial sexually transmitted infection (STI) worldwide. Diagnosis relies on nucleic acid amplification techniques, such as PCR, which does not distinguish between viable pathogens and residual bacterial DNA, leading to potential overdiagnosis and overtreatment. PCR with confirmation of pathogen viability has not been widely explored in the STI field.
View Article and Find Full Text PDFActa Biomater
January 2025
Department of Chemistry, Northeast Normal University, Changchun 130024, P. R. China. Electronic address:
The management of bacterial wounds presents a significant challenge in the field of medicine and poses a grave threat to public health. Traditional gauze materials exhibit limited efficacy in treating bacterial infection wounds, while antibiotics demonstrate cytotoxicity and resistance. Therefore, in this study, the peptide biomimetic polymer (PAL-BA) was designed and served as the antibacterial framework for constructing an antibiotic drug-free antibacterial hydrogel dressing through a Schiff base reaction with oxidized hyaluronic acid (OHA).
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