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Evaluation of mitochondrial redox status and energy metabolism of X-irradiated HeLa cells by LC/UV, LC/MS/MS and ESR.
Free Radic Res
June 2018
a Laboratory of Radiation Biology, Department of Applied Veterinary Sciences, Faculty of Veterinary Medicine , Hokkaido University, Sapporo , Japan.
To evaluate the metabolic responses in tumour cells exposed to ionizing radiation, oxygen consumption rate (OCR), cellular lipid peroxidation, cellular energy status (intracellular nucleotide pool and ATP production), and mitochondrial reactive oxygen species (ROS), semiquinone (SQ), and iron-sulphur (Fe-S) cluster levels were evaluated in human cervical carcinoma HeLa cells at 12 and 24 h after X-irradiation. LC/MS/MS analysis showed that levels of 8-iso PGF and 5-iPF-VI, lipid peroxidation products of membrane arachidonic acids, were not altered significantly in X-irradiated cells, although mitochondrial ROS levels and OCR significantly increased in the cells at 24 h after irradiation. LC/UV analysis revealed that intracellular AMP, ADP, and ATP levels increased significantly after X-irradiation, but adenylate energy charge (adenylate energy charge (AEC) = [ATP + 0.
View Article and Find Full Text PDFPostirradiation electron transfer vs differential radical decay in X-irradiated DNA and its mixtures with additives. Electron spin resonance spectroscopy in LiBr glass at low temperatures.
J Phys Chem B
August 2006
Fachrichtung Biophysik, Universität des Saarlandes, Klinikum Bau 76, 66421 Homburg, Germany.
Free radical formation in DNA and in colyophilized mixtures of DNA with the additives mitoxantrone and riboflavin was monitored after X-ray irradiation in frozen aqueous glasses (7 M LiBr/D2O) at 77 K by electron spin resonance (ESR) spectroscopy. Specifically, the postirradiation time course at 77 K of the respective free radical intensity residing on DNA or on the additive was probed in order to test the hypothesis of electron transfer from DNA, e.g.
View Article and Find Full Text PDFOptically detected ESR and low magnetic field signals from spin triads: 2-imidazoline-1-oxyl derivatives in X-irradiated alkane liquids as a method to study three-spin systems.
J Am Chem Soc
March 2004
Institute of Chemical Kinetics and Combustion SB RAS, Institutskaya 3, Novosibirsk 630090, Russia.
This contribution reports the design and synthesis of a series of spin-labeled charge acceptors to produce three-spin systems of "radical ion/biradical ion" type in X-irradiated alkane liquids. This opens the way to study spin triads in experimental conditions, in which short-lived radical ion pairs are conventionally studied, thus offering optically detected techniques such as magneto-resonance OD ESR and level-crossing MARY spectroscopy. The structure of the synthesized 2-imidazoline-1-oxyl derivatives is A-Sp-R, where A is a positive or negative charge acceptor, R is a stable radical, and Sp is a hydrocarbon bridge.
View Article and Find Full Text PDFEffects of low dose radiation therapy on adjuvant induced arthritis in rats.
Int J Radiat Biol
August 2000
Department of Radiotherapy and Radiooncology, University of Leipzig, Germany.
Purpose: Substantial clinical evidence shows the efficacy of low dose radiotherapy (RT) in the treatment of painful osteoarthritis. Experimental investigations into these empirically clinical observations remain scarce. This study investigated in vivo the effects of daily 5 x 1.
View Article and Find Full Text PDFSpin transfer from protein to DNA in X-irradiated 'dry' and hydrated chromatin: an electron spin resonance investigation of spectral components between 77 K and room temperature.
Int J Radiat Biol
August 2000
Fachrichtung Biophysik und Physikalische Grundlagen der Medizin, Universität des Saarlandes, Homburg/Saar, Germany.
Purpose: To investigate the potential total and relative free-radical transfer from histones to DNA in X-irradiated chromatin, by analysing the relative contributions of individual radicals from protein and from DNA.
Materials And Methods: Chromatin was isolated from calf thymus, freeze-dried and either used as such or after equilibration at 76% relative humidity. A mixture of histones was purchased.
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